Detailed information of TA system TA222614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 31224..31750
• Replicon: plasmid pPA_DBM3797_2
• Accession: NZ_CP086135
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7X2SUG9
• Locus tag: LKW31_RS21800
• Protein ID: WP_033759930.1
• Coordinates: 31224..31511 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8X8DPB7
• Locus tag: LKW31_RS21805
• Protein ID: WP_010257033.1
• Coordinates: 31511..31750 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS21775 (LKW31_21800)	27126..28151	-	1026	WP_010671942.1	alpha/beta hydrolase	-
LKW31_RS21780 (LKW31_21805)	28220..29344	-	1125	WP_228312816.1	MFS transporter	-
LKW31_RS21785 (LKW31_21810)	29547..30443	+	897	WP_010257019.1	LysR family transcriptional regulator	-
LKW31_RS21790 (LKW31_21815)	30507..30683	-	177	WP_010257023.1	KGG domain-containing protein	-
LKW31_RS21795 (LKW31_21820)	30883..31179	+	297	WP_039387382.1	hypothetical protein	-
LKW31_RS21800 (LKW31_21825)	31224..31511	-	288	WP_033759930.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKW31_RS21805 (LKW31_21830)	31511..31750	-	240	WP_010257033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LKW31_RS21810 (LKW31_21835)	32110..34083	+	1974	WP_191914328.1	methyl-accepting chemotaxis protein	-
LKW31_RS21815 (LKW31_21840)	34188..35204	+	1017	WP_010257039.1	NAD(P)-dependent alcohol dehydrogenase	-
LKW31_RS21820 (LKW31_21845)	35254..35922	-	669	WP_031292291.1	DUF2268 domain-containing putative Zn-dependent protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..182426	182426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-77)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11076.95 Da
• Isoelectric Point: 10.3173

>T222614 WP_033759930.1 NZ_CP086135:c31511-31224 [Pantoea agglomerans]
MTWQLEFDQRALKEWRKLGPTVREQLKKKLAECLESPRIEANKLSGMPDCYKIKLKSSGYRLVYQVIDDRVVVFVVAVGK
RERSEVYSAASRRLS

Nucleotide

• Length: 288 bp

>T222614 NZ_CP117971:c4431900-4431613 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9002.33 Da
• Isoelectric Point: 4.3410

>AT222614 WP_010257033.1 NZ_CP086135:c31750-31511 [Pantoea agglomerans]
MGTINIRIDDDLKTRSYAALEKMGVTPSDALRLMLEYIADNERLPFKQTLLSDEDAALVDIVKERLRNPEPVRVTLDDL

Nucleotide

• Length: 240 bp

>AT222614 NZ_CP117971:c4431626-4431324 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A7X2SUG9