Detailed information of TA system TA222613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 536983..537893
• Replicon: plasmid pPA_DBM3797_1
• Accession: NZ_CP086134
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LKW31_RS21595
• Protein ID: WP_228312741.1
• Coordinates: 537423..537893 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LKW31_RS21590
• Protein ID: WP_022624589.1
• Coordinates: 536983..537426 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS21565 (LKW31_21590)	533097..533996	-	900	WP_228312738.1	alpha/beta hydrolase	-
LKW31_RS21570 (LKW31_21595)	534047..534661	-	615	WP_228312739.1	TetR/AcrR family transcriptional regulator	-
LKW31_RS21575 (LKW31_21600)	534875..535292	+	418	Protein_512	LysR substrate-binding domain-containing protein	-
LKW31_RS21580 (LKW31_21605)	535327..536316	-	990	WP_228312740.1	aldo/keto reductase	-
LKW31_RS21585 (LKW31_21610)	536342..536567	-	226	Protein_514	aldo/keto reductase	-
LKW31_RS21590 (LKW31_21615)	536983..537426	+	444	WP_022624589.1	DUF2384 domain-containing protein	Antitoxin
LKW31_RS21595 (LKW31_21620)	537423..537893	+	471	WP_228312741.1	RES family NAD+ phosphorylase	Toxin
LKW31_RS21600 (LKW31_21625)	538065..539096	+	1032	WP_031594315.1	AI-2E family transporter	-
LKW31_RS21605 (LKW31_21630)	539085..541814	-	2730	WP_228312742.1	cation-transporting P-type ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..555522	555522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (32-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17528.97 Da
• Isoelectric Point: 4.7790

>T222613 WP_228312741.1 NZ_CP086134:537423-537893 [Pantoea agglomerans]
MIFYRLTKTKYLSTAWTGFGAKEAGGRWNSIGISMVYVSETASLTLLETLVHLHSAHILDAFTLLRIDVPDALIQTADIT
ELPANWADEDAPAELADYGDAWCSACDAVALRVPSALSPVEYNYLLNPEHPEFFRIIQQAEAIPFRFDRRLKPDRK

Nucleotide

• Length: 471 bp

>T222613 NZ_CP117971:4123206-4123484 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16474.91 Da
• Isoelectric Point: 9.9250

>AT222613 WP_022624589.1 NZ_CP086134:536983-537426 [Pantoea agglomerans]
MKTFSFSANKTRPPRLWQAAGLNNADGVALLGQINEGLDGNVAHLIVNWAKITQNDLRKMSGIPSTTFSRSVKAKFSAEQ
SERLVRIIRVIDRAVELFEGDKDEALKWLNEPNRALSWKMPAELMASETGAYEVMKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT222613 NZ_CP117971:4122943-4123203 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA