Detailed information of TA system TA222606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3388029..3388660
• Replicon: chromosome
• Accession: NZ_CP086133
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7X5MR53
• Locus tag: LKW31_RS15980
• Protein ID: WP_010253953.1
• Coordinates: 3388481..3388660 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8X8DTB6
• Locus tag: LKW31_RS15975
• Protein ID: WP_010253955.1
• Coordinates: 3388029..3388454 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS15955 (3384750)	3384750..3385259	-	510	WP_010253965.1	phenolic acid decarboxylase	-
LKW31_RS15960 (3385365)	3385365..3386264	+	900	WP_172608183.1	LysR family transcriptional regulator	-
LKW31_RS15965 (3386692)	3386692..3387621	-	930	WP_191913914.1	Rpn family recombination-promoting nuclease/putative transposase	-
LKW31_RS15970 (3387858)	3387858..3387975	-	118	Protein_3114	subtype I-E CRISPR-associated endonuclease Cas1	-
LKW31_RS15975 (3388029)	3388029..3388454	-	426	WP_010253955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LKW31_RS15980 (3388481)	3388481..3388660	-	180	WP_010253953.1	type II toxin-antitoxin system HicA family toxin	Toxin
LKW31_RS15985 (3388843)	3388843..3390267	-	1425	WP_010253950.1	dihydrolipoyl dehydrogenase	-
LKW31_RS15990 (3390428)	3390428..3392329	-	1902	WP_010671838.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6467.52 Da
• Isoelectric Point: 10.7891

>T222606 WP_010253953.1 NZ_CP086133:c3388660-3388481 [Pantoea agglomerans]
MDSSTLISEMKADGWVLTGINGSHHHFTHPTKPGLVTVPHPKKDLPIGTVKSIRKQARI

Nucleotide

• Length: 180 bp

>T222606 NZ_CP117971:2657625-2658947 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15422.60 Da
• Isoelectric Point: 4.7662

>AT222606 WP_010253955.1 NZ_CP086133:c3388454-3388029 [Pantoea agglomerans]
MFYPIAIEAGDHEHAYGVIVPDLPGCFSAGDTLDEAIKNAKEAITGHIELCVELGHEIPAVSTIETLATNPDYQGYIWAL
VDVDVIRLLGGSEKINVTLPRSLIDRIDRCVASHPEYKSRSGFLAQVALERITAPINRPQK

Nucleotide

• Length: 426 bp

>AT222606 NZ_CP117971:2657359-2657625 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7X5MR53