Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3114688..3115305 Replicon chromosome
Accession NZ_CP086133
Organism Pantoea agglomerans strain DBM 3797

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag LKW31_RS14675 Protein ID WP_003850458.1
Coordinates 3115090..3115305 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag LKW31_RS14670 Protein ID WP_003850455.1
Coordinates 3114688..3115065 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKW31_RS14640 (3110995) 3110995..3111249 + 255 WP_010671219.1 type B 50S ribosomal protein L31 -
LKW31_RS14645 (3111261) 3111261..3111401 + 141 WP_010255896.1 type B 50S ribosomal protein L36 -
LKW31_RS14650 (3111447) 3111447..3112325 - 879 WP_228312256.1 metal ABC transporter substrate-binding protein -
LKW31_RS14655 (3112341) 3112341..3113180 - 840 WP_187512285.1 metal ABC transporter permease -
LKW31_RS14660 (3113177) 3113177..3113845 - 669 WP_228312257.1 ABC transporter ATP-binding protein -
LKW31_RS14665 (3114187) 3114187..3114540 + 354 WP_010255885.1 hypothetical protein -
LKW31_RS14670 (3114688) 3114688..3115065 + 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
LKW31_RS14675 (3115090) 3115090..3115305 + 216 WP_003850458.1 HHA domain-containing protein Toxin
LKW31_RS14685 (3115712) 3115712..3116023 + 312 WP_228312258.1 MGMT family protein -
LKW31_RS14690 (3116063) 3116063..3116620 - 558 WP_010255881.1 YbaY family lipoprotein -
LKW31_RS14695 (3116811) 3116811..3117674 + 864 WP_010255880.1 acyl-CoA thioesterase II -
LKW31_RS14700 (3117727) 3117727..3119013 - 1287 WP_010255877.1 ammonium transporter AmtB -
LKW31_RS14705 (3119048) 3119048..3119386 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T222605 WP_003850458.1 NZ_CP086133:3115090-3115305 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T222605 NZ_CP117971:2603072-2603359 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT222605 WP_003850455.1 NZ_CP086133:3114688-3115065 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT222605 NZ_CP117971:2602833-2603072 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References