Detailed information of TA system TA222599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 658101..658693
• Replicon: chromosome
• Accession: NZ_CP086133
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LKW31_RS03150
• Protein ID: WP_029519675.1
• Coordinates: 658101..658355 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LKW31_RS03155
• Protein ID: WP_010256658.1
• Coordinates: 658352..658693 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS03115 (653194)	653194..653424	+	231	WP_010256641.1	DUF2732 family protein	-
LKW31_RS03120 (653424)	653424..653651	+	228	WP_228312413.1	TraR/DksA family transcriptional regulator	-
LKW31_RS03125 (653648)	653648..654511	+	864	WP_228312414.1	DNA adenine methylase	-
LKW31_RS03130 (654508)	654508..656754	+	2247	WP_228312415.1	replication endonuclease	-
LKW31_RS03135 (656959)	656959..657150	+	192	WP_010256650.1	hypothetical protein	-
LKW31_RS03140 (657166)	657166..657402	+	237	WP_228312416.1	DinI family protein	-
LKW31_RS03145 (657477)	657477..657734	+	258	WP_228312417.1	hypothetical protein	-
LKW31_RS03150 (658101)	658101..658355	+	255	WP_029519675.1	type II toxin-antitoxin system HicA family toxin	Toxin
LKW31_RS03155 (658352)	658352..658693	+	342	WP_010256658.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LKW31_RS03160 (658766)	658766..659683	+	918	WP_228312418.1	nucleotide-binding protein	-
LKW31_RS03165 (659735)	659735..660769	-	1035	WP_228312419.1	phage portal protein	-
LKW31_RS03170 (660769)	660769..662532	-	1764	WP_010255070.1	terminase ATPase subunit family protein	-
LKW31_RS03175 (662677)	662677..663525	+	849	WP_115759155.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	642556..716949	74393

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9481.99 Da
• Isoelectric Point: 11.3526

>T222599 WP_029519675.1 NZ_CP086133:658101-658355 [Pantoea agglomerans]
MNKRHQKTLSDVFARPVSGSIKWSDIESLFIALGAEVHEREGSRIAVLLKGQKKIFHRPHPRPTTDKGAVNSIRVWLDSL
GIRP

Nucleotide

• Length: 255 bp

>T222599 NZ_CP117971:1061693-1061989 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12527.13 Da
• Isoelectric Point: 4.6017

>AT222599 WP_010256658.1 NZ_CP086133:658352-658693 [Pantoea agglomerans]
MNNTLKIDGHLAVITFDPEIEMFRGEFVGLNGGADFYAYSVEELKKEGSISLAVFLDECRKDDIDPYKTFSGKVTTRLTP
ERHQALAVTAQAHGVSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT222599 NZ_CP117971:1061991-1062386 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA