Detailed information of TA system TA222595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4558252..4558768
• Replicon: chromosome
• Accession: NZ_CP086128
• Organism: Klebsiella pneumoniae strain MIN-109

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LLZ91_RS22085
• Protein ID: WP_234573853.1
• Coordinates: 4558252..4558536 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LLZ91_RS22090
• Protein ID: WP_002886901.1
• Coordinates: 4558526..4558768 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ91_RS22060 (LLZ91_22090)	4553668..4553931	-	264	WP_025368518.1	PTS sugar transporter subunit IIB	-
LLZ91_RS22065 (LLZ91_22095)	4554061..4554234	+	174	WP_002886906.1	hypothetical protein	-
LLZ91_RS22070 (LLZ91_22100)	4554237..4554980	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LLZ91_RS22075 (LLZ91_22105)	4555337..4557475	+	2139	WP_004222153.1	anaerobic ribonucleoside-triphosphate reductase	-
LLZ91_RS22080 (LLZ91_22110)	4557784..4558248	+	465	WP_004192393.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LLZ91_RS22085 (LLZ91_22115)	4558252..4558536	-	285	WP_234573853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LLZ91_RS22090 (LLZ91_22120)	4558526..4558768	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LLZ91_RS22095 (LLZ91_22125)	4558846..4560756	-	1911	WP_004152270.1	PRD domain-containing protein	-
LLZ91_RS22100 (LLZ91_22130)	4560779..4561933	-	1155	WP_130976729.1	lactonase family protein	-
LLZ91_RS22105 (LLZ91_22135)	4562000..4562740	-	741	WP_004186692.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11125.95 Da
• Isoelectric Point: 10.3787

>T222595 WP_234573853.1 NZ_CP086128:c4558536-4558252 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKIQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T222595 NZ_CP117971:c370364-369987 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGTCGGCCAGTTCACGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGGC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCAGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT222595 WP_002886901.1 NZ_CP086128:c4558768-4558526 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT222595 NZ_CP117971:c370822-370454 [Escherichia coli]
GTGTCAGACACGCTCCCTGAGACAAATAACCCCAACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGCGAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGGCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0