Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4435510..4436320 Replicon chromosome
Accession NZ_CP086128
Organism Klebsiella pneumoniae strain MIN-109

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LLZ91_RS21525 Protein ID WP_004178461.1
Coordinates 4435510..4436043 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LLZ91_RS21530 Protein ID WP_002887278.1
Coordinates 4436054..4436320 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLZ91_RS21520 (LLZ91_21545) 4434341..4435462 + 1122 WP_016946712.1 cupin domain-containing protein -
LLZ91_RS21525 (LLZ91_21550) 4435510..4436043 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LLZ91_RS21530 (LLZ91_21555) 4436054..4436320 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LLZ91_RS21535 (LLZ91_21560) 4436423..4437856 - 1434 WP_200779552.1 Cu(+)/Ag(+) sensor histidine kinase -
LLZ91_RS21540 (LLZ91_21565) 4437846..4438529 - 684 WP_234573834.1 copper response regulator transcription factor CusR -
LLZ91_RS21545 (LLZ91_21570) 4438702..4440087 + 1386 WP_032413315.1 efflux transporter outer membrane subunit -
LLZ91_RS21550 (LLZ91_21575) 4440105..4440449 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T222594 WP_004178461.1 NZ_CP086128:c4436043-4435510 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T222594 NZ_CP117970:2805351-2806004 [Enterococcus faecalis]
TTGATTGGACTGTTGTTGGTGGATTTTATTAGTAAAGAACTCTATTTTGAAGCATTTAATTTTTCGAACGAATTAATAAA
AGAAGTATCTTACTACTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
ATGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATCTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAAGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAGGTTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACTCATACTTTTTCTGATATTCTTTTGAAAAATGGTTACTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGTATGTTAATGGCAAATGATCAAGCATTGTTTTACGCATTAAAAAAATTT
TATTCATTCGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATCGAGCATCTTATGTA
TGTAAATAATTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTCAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT222594 WP_002887278.1 NZ_CP086128:c4436320-4436054 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT222594 NZ_CP117970:2804971-2805354 [Enterococcus faecalis]
GTGCAACCATTAGCAAAAAGACTACAGCTTTTAAGAGAAGAAAAAGAATGGACAAAAACATATGTTGCAAAACAACTAGG
TTTAAATAACTTAGGAACCTATGCAAACTGGGAATATGGAACACGTGAACCAGATTCGGAAATGCTATCAAAAATTGCTT
CCTTATATGATGTATCTACCGATTTTTTACTAGGTAGAACAGAGGAAAGAATACATCTAAACGACAAGGAAAAAATTGGA
AAAAATATCATTTCTCATTTTCGTTTAAATACTTCTGATATGGACATAGAAGATATAGAAGAATTAGAAGAAGAATTAAT
CGACTTTCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGGAACGTAACAAAAAAAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References