Detailed information of TA system TA222592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2579682..2581270
• Replicon: chromosome
• Accession: NZ_CP086128
• Organism: Klebsiella pneumoniae strain MIN-109

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LLZ91_RS12665
• Protein ID: WP_234573684.1
• Coordinates: 2579682..2581004 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LLZ91_RS12670
• Protein ID: WP_002904790.1
• Coordinates: 2581004..2581270 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ91_RS12650 (LLZ91_12665)	2574738..2577239	+	2502	WP_234573682.1	fimbria/pilus outer membrane usher protein	-
LLZ91_RS12655 (LLZ91_12670)	2577227..2578231	+	1005	WP_016946380.1	fimbrial protein	-
LLZ91_RS12660 (LLZ91_12675)	2578373..2579665	+	1293	WP_234573683.1	glycoside hydrolase family 10 protein	-
LLZ91_RS12665 (LLZ91_12680)	2579682..2581004	-	1323	WP_234573684.1	type II toxin-antitoxin system HipA family toxin	Toxin
LLZ91_RS12670 (LLZ91_12685)	2581004..2581270	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LLZ91_RS12675 (LLZ91_12690)	2581778..2582104	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
LLZ91_RS12680 (LLZ91_12695)	2582101..2582601	-	501	WP_234573685.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LLZ91_RS12685 (LLZ91_12700)	2582579..2583958	-	1380	WP_234573686.1	nucleobase:cation symporter-2 family protein	-
LLZ91_RS12690 (LLZ91_12705)	2584304..2585458	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49154.48 Da
• Isoelectric Point: 7.8920

>T222592 WP_234573684.1 NZ_CP086128:c2581004-2579682 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITANAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T222592 NZ_CP117970:c2475095-2474979 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT222592 WP_002904790.1 NZ_CP086128:c2581270-2581004 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT222592 NZ_CP117970:2474863-2475068 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8