Detailed information of TA system TA022259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 4245904..4246531
• Replicon: chromosome
• Accession: NC_009633
• Organism: Alkaliphilus metalliredigens QYMF

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A6TVK6
• Locus tag: AMET_RS20335
• Protein ID: WP_012065172.1
• Coordinates: 4245904..4246254 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A6TVK7
• Locus tag: AMET_RS20340
• Protein ID: WP_012065173.1
• Coordinates: 4246256..4246531 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMET_RS20320	4241574..4242680	-	1107	WP_012065169.1	polysaccharide pyruvyl transferase CsaB	-
AMET_RS20325	4242673..4244844	-	2172	WP_012065170.1	hypothetical protein	-
AMET_RS24585	4245167..4245700	-	534	WP_012065171.1	hypothetical protein	-
AMET_RS20335	4245904..4246254	-	351	WP_012065172.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AMET_RS20340	4246256..4246531	-	276	WP_012065173.1	ribbon-helix-helix protein, CopG family	Antitoxin
AMET_RS20345	4246716..4247891	-	1176	WP_012065174.1	alanine racemase	-
AMET_RS20350	4247904..4248563	-	660	WP_012065175.1	outer membrane lipoprotein-sorting protein	-
AMET_RS20355	4248663..4249031	-	369	WP_012065176.1	holo-ACP synthase	-
AMET_RS20360	4249203..4249823	-	621	WP_012065177.1	hypothetical protein	-
AMET_RS20365	4250079..4251041	+	963	WP_157047320.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12863.91 Da
• Isoelectric Point: 5.1568

>T22259 WP_012065172.1 NC_009633:c4246254-4245904 [Alkaliphilus metalliredigens QYMF]
VVVKRGDIYYADLSPVIGSEQGGVRPVLIIQNDIGNRYSPTVIVAAITSQINKAKLPTHIEITASDYGLPKDSVVLLEQI
RTIDKRRLEEKVGHFDEEMMIKVNEALLISCGLIDF

Nucleotide

• Length: 351 bp

>T22259 NC_009633:c4246254-4245904 [Alkaliphilus metalliredigens QYMF]
GTGGTTGTCAAAAGAGGAGACATCTATTATGCAGATCTAAGTCCTGTCATTGGATCGGAGCAAGGGGGCGTTAGGCCGGT
TTTAATCATTCAAAATGATATCGGAAACCGGTACAGTCCAACGGTGATTGTTGCAGCAATTACGTCTCAAATTAACAAGG
CAAAGTTACCAACTCATATTGAAATCACTGCCTCTGACTATGGTTTGCCTAAAGACTCTGTTGTTTTACTAGAACAAATC
AGGACGATTGATAAAAGACGATTAGAAGAAAAAGTCGGACACTTTGATGAGGAAATGATGATTAAAGTAAATGAAGCGTT
ATTAATTAGCTGTGGATTGATAGATTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10548.12 Da
• Isoelectric Point: 4.3356

>AT22259 WP_012065173.1 NC_009633:c4246531-4246256 [Alkaliphilus metalliredigens QYMF]
VADSKRIMISLPDSLLQEVDGIVSMEQTNRSEFIREAMKLYIRERNKMELREKMITGYQEMGYINLAIAELGLSLDISSL
EHYETQMAECE

Nucleotide

• Length: 276 bp

>AT22259 NC_009633:c4246531-4246256 [Alkaliphilus metalliredigens QYMF]
GTGGCCGACTCTAAACGAATTATGATTAGTCTTCCGGACAGTCTTTTACAAGAGGTAGATGGGATTGTTTCTATGGAACA
AACAAACCGAAGTGAATTTATTCGTGAAGCAATGAAACTCTACATTAGGGAGAGAAATAAAATGGAGTTAAGAGAGAAGA
TGATCACTGGGTATCAAGAAATGGGATATATTAACTTAGCCATTGCAGAACTTGGATTGAGTTTAGATATTTCTTCATTA
GAGCACTATGAGACTCAAATGGCGGAGTGTGAGTAG

Structures

Toxin

• AlphaFold DB: A6TVK6

Antitoxin

• AlphaFold DB: A6TVK7