222577

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4717465..4718073 Replicon chromosome
Accession NZ_CP086122
Organism Pseudomonas aeruginosa strain MIN-155

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag LLZ84_RS21830 Protein ID WP_003114156.1
Coordinates 4717465..4717812 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag LLZ84_RS21835 Protein ID WP_003114155.1
Coordinates 4717822..4718073 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLZ84_RS21800 (LLZ84_21805) 4712824..4713096 + 273 WP_010895521.1 cysteine-rich CWC family protein -
LLZ84_RS21805 (LLZ84_21810) 4713096..4713788 + 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
LLZ84_RS21810 (LLZ84_21815) 4713924..4714967 + 1044 WP_003098363.1 L,D-transpeptidase -
LLZ84_RS21815 (LLZ84_21820) 4715047..4715784 + 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
LLZ84_RS21820 (LLZ84_21825) 4716236..4717138 + 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
LLZ84_RS21830 (LLZ84_21835) 4717465..4717812 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LLZ84_RS21835 (LLZ84_21840) 4717822..4718073 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LLZ84_RS21840 (LLZ84_21845) 4718287..4719270 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
LLZ84_RS21845 (LLZ84_21850) 4719270..4720562 - 1293 WP_003115206.1 hypothetical protein -
LLZ84_RS21850 (LLZ84_21855) 4720792..4722066 - 1275 WP_010895520.1 zonular occludens toxin family protein -
LLZ84_RS21855 (LLZ84_21860) 4722070..4722426 - 357 WP_003114150.1 DUF2523 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4708361..4739641 31280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T222577 WP_003114156.1 NZ_CP086122:c4717812-4717465 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T222577 NZ_CP117962:4233587-4233690 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT222577 WP_003114155.1 NZ_CP086122:c4718073-4717822 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT222577 NZ_CP117962:c4233728-4233457 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References