Detailed information of TA system TA222576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4397670..4398928
• Replicon: chromosome
• Accession: NZ_CP086122
• Organism: Pseudomonas aeruginosa strain MIN-155

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: LLZ84_RS20245
• Protein ID: WP_003112528.1
• Coordinates: 4397670..4398425 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LLZ84_RS20250
• Protein ID: WP_003086329.1
• Coordinates: 4398560..4398928 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ84_RS20235 (LLZ84_20235)	4392843..4395386	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
LLZ84_RS20240 (LLZ84_20240)	4395541..4396902	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
LLZ84_RS20245 (LLZ84_20245)	4397670..4398425	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
LLZ84_RS20250 (LLZ84_20250)	4398560..4398928	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LLZ84_RS20255 (LLZ84_20255)	4399001..4400287	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
LLZ84_RS20260 (LLZ84_20260)	4400343..4401932	-	1590	WP_010895535.1	aldehyde dehydrogenase family protein	-
LLZ84_RS20265 (LLZ84_20265)	4402011..4402487	+	477	WP_003112531.1	hypothetical protein	-
LLZ84_RS20270 (LLZ84_20270)	4402490..4402765	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T222576 WP_003112528.1 NZ_CP086122:c4398425-4397670 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T222576 NZ_CP117962:c4080001-4079627 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT222576 WP_003086329.1 NZ_CP086122:c4398928-4398560 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT222576 NZ_CP117962:c4080458-4080090 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7