Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 419996..421475 Replicon plasmid pSMED02
Accession NC_009621
Organism Sinorhizobium medicae WSM419

Toxin (Protein)


Gene name rlegT Uniprot ID A0A508XC36
Locus tag SMED_RS27095 Protein ID WP_011970323.1
Coordinates 420606..421475 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A6G1WUK8
Locus tag SMED_RS27090 Protein ID WP_011970322.1
Coordinates 419996..420613 (+) Length 206 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMED_RS27070 415102..415872 - 771 WP_208870265.1 alpha/beta fold hydrolase -
SMED_RS27075 416125..416393 - 269 Protein_386 hypothetical protein -
SMED_RS27080 416613..418256 - 1644 Protein_387 ISL3 family transposase -
SMED_RS27085 418647..419801 + 1155 WP_208870264.1 hypothetical protein -
SMED_RS27090 419996..420613 + 618 WP_011970322.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
SMED_RS27095 420606..421475 + 870 WP_011970323.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
SMED_RS27100 421566..422507 - 942 WP_011970324.1 ribokinase -
SMED_RS27105 422750..423856 - 1107 WP_011970325.1 LacI family transcriptional regulator -
SMED_RS27110 424100..424945 + 846 WP_011970326.1 ABC transporter substrate-binding protein -
SMED_RS27115 425015..425938 + 924 WP_011970327.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..1245408 1245408
- flank IS/Tn - - 416697..418307 1610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-262)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(16-56)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32476.29 Da        Isoelectric Point: 5.2692

>T22255 WP_011970323.1 NC_009621:420606-421475 [Sinorhizobium medicae WSM419]
MAKEIRNVGASVRARLLQLAKASGQSFDVVLTRFALERLLFRLSRSPHAGRFVLKGAMLMMSWFDDPHRGTRDLDLLGFG
NPEPDPMLATFREILAQEVDDGVMFDADTLRVDRIREELEYGGLRLRAVASISGARINLTIDIGFGDALEPGAEMLDYPS
MLDFPAPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFDDDRLARAIAATFERRETPIPEDLPDALTD
AFAKDEQKQRQWRAFVEGVAHNPGDLTNVIAEIASFLMPHAIAAAKLGK

Download         Length: 870 bp

>T22255 NC_009621:420606-421475 [Sinorhizobium medicae WSM419]
ATGGCTAAGGAGATCAGAAATGTTGGTGCCTCGGTGCGCGCCCGCCTGCTGCAACTCGCCAAGGCAAGCGGGCAAAGCTT
CGATGTGGTTCTGACGCGTTTTGCCCTTGAGCGGTTACTGTTCCGGCTCAGCCGGTCACCCCATGCCGGTCGTTTCGTGC
TGAAGGGCGCCATGCTGATGATGAGCTGGTTCGACGATCCGCACCGCGGAACGCGCGACCTCGACCTTCTGGGCTTTGGT
AATCCCGAGCCGGACCCGATGCTGGCGACCTTTCGGGAAATCCTGGCGCAAGAGGTTGATGATGGCGTCATGTTCGATGC
CGACACCCTGCGGGTGGATCGCATCCGTGAGGAGCTGGAATATGGTGGGCTCCGGCTACGGGCGGTCGCTTCGATCAGCG
GCGCCCGGATCAACCTGACGATCGACATCGGCTTCGGCGATGCGCTAGAGCCCGGGGCGGAGATGTTGGACTATCCTTCA
ATGCTGGATTTCCCGGCGCCACGACTCCGGGCCTATGCGCGGGAGACAGTCATCGCCGAGAAGTTCCAGGCGATGGTCAT
GCTCGGGCGTGTGAACAGCCGGATGAAGGACTTCTATGACATCTGGATTCTCAGCAGGTCTTTTGATTTCGATGATGACA
GGCTGGCGCGCGCAATCGCCGCCACCTTCGAGCGCCGCGAAACGCCGATCCCTGAAGACCTACCGGATGCTTTGACGGAT
GCTTTTGCTAAGGACGAGCAAAAGCAACGGCAATGGCGCGCTTTTGTCGAAGGCGTTGCGCACAATCCGGGGGATCTGAC
GAATGTCATCGCGGAGATTGCTTCGTTCCTGATGCCGCACGCTATTGCAGCCGCGAAGCTGGGAAAATAA

Antitoxin


Download         Length: 206 a.a.        Molecular weight: 22344.87 Da        Isoelectric Point: 10.2490

>AT22255 WP_011970322.1 NC_009621:419996-420613 [Sinorhizobium medicae WSM419]
MSGSVTQRQIARTVLIERGIARLAELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAELDTNHSLAETAKRVPKAVVCL
VSALAFHGLTDQLPRHVWLAIGRKDWAPKLDGTPIRIVRFTDSLLNDGVEIHVVEGVPVKVFGVAKTIADCFRHRSKIGL
SVAIEGLQEALRQRKATPSDIVNQAERGGVGTVIRPYLEALTVNG

Download         Length: 618 bp

>AT22255 NC_009621:419996-420613 [Sinorhizobium medicae WSM419]
ATGTCTGGCTCCGTCACTCAACGTCAAATCGCCCGGACCGTGCTCATCGAGCGCGGAATAGCACGCCTGGCGGAGCTTCG
GAACGCTGGTGTGACGGCGGCAACCATGAGCCGGATGGAACGGGATGGCGAGGTGCTGCGACTTGCCCGTGGCCTCTACC
AACTTCCCGACGCCGAGCTCGATACCAACCATAGTCTGGCAGAGACCGCCAAGCGTGTTCCGAAAGCCGTTGTCTGTCTT
GTTTCAGCTCTCGCATTCCACGGCCTGACCGATCAGCTGCCGAGACACGTGTGGCTTGCCATAGGCCGCAAAGACTGGGC
GCCGAAACTAGATGGCACACCTATTCGTATCGTGCGCTTTACGGACAGCCTGCTTAACGATGGTGTCGAAATCCATGTGG
TCGAAGGCGTTCCTGTAAAGGTCTTTGGAGTCGCCAAAACAATTGCCGATTGCTTCCGCCATCGCAGCAAGATCGGTCTT
TCTGTGGCGATCGAGGGACTTCAGGAAGCACTGCGGCAGCGCAAGGCTACGCCCAGCGACATCGTCAACCAGGCCGAACG
TGGTGGTGTCGGCACCGTGATCCGCCCCTATCTCGAGGCATTGACCGTCAATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A508XC36

Antitoxin

Source ID Structure
AlphaFold DB A0A6G1WUK8

References