Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 143173..143729 Replicon plasmid pSR-2
Accession NZ_CP086113
Organism Skermanella rosea strain KEMB 2255-458

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag JL101_RS32015 Protein ID WP_203102656.1
Coordinates 143394..143729 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag JL101_RS32010 Protein ID WP_203102655.1
Coordinates 143173..143397 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL101_RS31995 (JL101_032075) 138672..140180 + 1509 WP_203102653.1 hypothetical protein -
JL101_RS32000 (JL101_032080) 140378..141961 + 1584 WP_203102654.1 sulfatase-like hydrolase/transferase -
JL101_RS32005 (JL101_032085) 142030..142482 - 453 WP_267133578.1 transposase -
JL101_RS32010 (JL101_032090) 143173..143397 + 225 WP_203102655.1 antitoxin MazE family protein Antitoxin
JL101_RS32015 (JL101_032095) 143394..143729 + 336 WP_203102656.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JL101_RS32020 (JL101_032100) 143849..143950 + 102 Protein_143 IS5/IS1182 family transposase -
JL101_RS32025 (JL101_032105) 144727..145824 + 1098 WP_203102658.1 calcium-binding protein -
JL101_RS32030 (JL101_032110) 145948..146088 - 141 WP_203102660.1 hypothetical protein -
JL101_RS32035 (JL101_032115) 146243..147016 - 774 WP_203102662.1 calcium-binding protein -
JL101_RS32040 (JL101_032120) 147359..148603 - 1245 WP_203102664.1 cytochrome P450 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hsiC1/vipB 1..529001 529001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

Antitoxin MazE-like

(6-68)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12075.95 Da        Isoelectric Point: 10.7568

>T222545 WP_203102656.1 NZ_CP086113:143394-143729 [Skermanella rosea]
VRRGDLVTIAGRIGGDYDGKPRPAVIIQSDDFPDTDSVTVSLLTSIGIDAPLLRLRIDPAPANNLRQTSWVMIDKIATVR
RRNIRAVFGRLSDAELVQVNRALPVFLGIAG

Download         Length: 336 bp

>T222545 NZ_CP117960:3326513-3326827 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8421.39 Da        Isoelectric Point: 5.6624

>AT222545 WP_203102655.1 NZ_CP086113:143173-143397 [Skermanella rosea]
MPSTVRHRVQTHRAQLRAQGLRPIQIWVPDVRSPTFKEEARRQSRAVAAAPDEADTMDFLEDIQDLGDDVGDRP

Download         Length: 225 bp

>AT222545 NZ_CP117960:3326824-3327240 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References