Detailed information of TA system TA222540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4131808..4132418
• Replicon: chromosome
• Accession: NZ_CP086111
• Organism: Skermanella rosea strain KEMB 2255-458

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JL101_RS19310
• Protein ID: WP_228434980.1
• Coordinates: 4132062..4132418 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JL101_RS19305
• Protein ID: WP_203097767.1
• Coordinates: 4131808..4132065 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL101_RS19275 (JL101_019315)	4127586..4128701	-	1116	WP_203097770.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
JL101_RS19280 (JL101_019320)	4128698..4129165	-	468	WP_203097846.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
JL101_RS19285 (JL101_019325)	4129308..4129508	-	201	WP_158046558.1	DUF465 domain-containing protein	-
JL101_RS19290 (JL101_019330)	4129706..4130791	+	1086	WP_203097769.1	ATP-dependent 6-phosphofructokinase	-
JL101_RS19295 (JL101_019335)	4130781..4131323	+	543	WP_228434978.1	TIGR02444 family protein	-
JL101_RS19300 (JL101_019340)	4131430..4131732	+	303	WP_203097768.1	hypothetical protein	-
JL101_RS19305 (JL101_019345)	4131808..4132065	+	258	WP_203097767.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JL101_RS19310 (JL101_019350)	4132062..4132418	+	357	WP_228434980.1	type II toxin-antitoxin system VapC family toxin	Toxin
JL101_RS19315 (JL101_019355)	4132481..4133107	-	627	WP_203097766.1	UbiX family flavin prenyltransferase	-
JL101_RS19320 (JL101_019360)	4133114..4133818	-	705	WP_203097765.1	DUF1013 domain-containing protein	-
JL101_RS19325 (JL101_019365)	4133978..4134985	-	1008	WP_203097764.1	NAD(P)H-quinone oxidoreductase	-
JL101_RS19330 (JL101_019370)	4135142..4135324	+	183	WP_203097763.1	DUF1192 domain-containing protein	-
JL101_RS19335 (JL101_019375)	4135355..4136071	-	717	WP_203097762.1	DUF599 domain-containing protein	-
JL101_RS19340 (JL101_019380)	4136089..4136319	-	231	WP_203097761.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-114)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12789.81 Da
• Isoelectric Point: 9.7567

>T222540 WP_228434980.1 NZ_CP086111:4132062-4132418 [Skermanella rosea]
MIVDTSAVIAILELEPEAADFAGLIAKAETPWISAVAVVEAGMLADSRRGPAGADELDRFLVRARFQIVPFDHVQARIAR
LAFRRYGKGRHPTSLNLGDCIAYSLSKASGKPLLSKRQ

Nucleotide

• Length: 357 bp

>T222540 NZ_CP117960:1631247-1631525 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9559.74 Da
• Isoelectric Point: 4.5854

>AT222540 WP_203097767.1 NZ_CP086111:4131808-4132065 [Skermanella rosea]
MPLDIKDPEADRLASELARRRGVSVAEAVLAALREQIRREDEKSNSSVTAGELLEIGKRYSSLPILDDRPEEEILGYDQQ
GLPRR

Nucleotide

• Length: 258 bp

>AT222540 NZ_CP117960:1630984-1631244 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA