Detailed information of TA system TA022254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1026227..1027104
• Replicon: plasmid pSMED01
• Accession: NC_009620
• Organism: Sinorhizobium medicae WSM419

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A508X590
• Locus tag: SMED_RS22700
• Protein ID: WP_012061472.1
• Coordinates: 1026604..1027104 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A508X7R7
• Locus tag: SMED_RS22695
• Protein ID: WP_012061471.1
• Coordinates: 1026227..1026598 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMED_RS22675	1022115..1022498	+	384	WP_018012062.1	RidA family protein	-
SMED_RS22680	1022515..1024503	+	1989	WP_012061468.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
SMED_RS22685	1024500..1025369	+	870	WP_012061469.1	hydroxymethylglutaryl-CoA lyase	-
SMED_RS22690	1025366..1026151	+	786	WP_012061470.1	crotonase/enoyl-CoA hydratase family protein	-
SMED_RS22695	1026227..1026598	+	372	WP_012061471.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
SMED_RS22700	1026604..1027104	+	501	WP_012061472.1	RES family NAD+ phosphorylase	Toxin
SMED_RS22705	1027229..1028599	-	1371	WP_012061473.1	FAD-binding oxidoreductase	-
SMED_RS22710	1028728..1029831	-	1104	WP_012061474.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SMED_RS22715	1029847..1030713	-	867	WP_012061475.1	sulfate ABC transporter permease subunit CysW	-
SMED_RS22720	1030703..1031569	-	867	WP_018209856.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1570951	1570951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-159)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18501.16 Da
• Isoelectric Point: 6.3284

>T22254 WP_012061472.1 NC_009620:1026604-1027104 [Sinorhizobium medicae WSM419]
VEALTLWRAFVPRWAHMPLSGAGAARFGGRWNPVGVPALYAARELSTAWAEYNQGFVQHPALIVQLELRDAVLADLTDFK
VLADLDVDETIHSCEWRDMLDKGAVPQTHQLRTALLARDYHGVIYPSFMSPGGTCVALWRWNGAKEPRLDVIDPEGRLPK
SPASWV

Nucleotide

• Length: 501 bp

>T22254 NC_009620:1026604-1027104 [Sinorhizobium medicae WSM419]
GTGGAAGCGCTAACGCTCTGGCGTGCCTTCGTACCGCGCTGGGCGCATATGCCGCTTTCCGGTGCAGGTGCCGCCCGTTT
CGGTGGCCGCTGGAATCCGGTCGGGGTACCTGCTCTTTATGCCGCGCGTGAACTTTCGACCGCCTGGGCGGAATACAATC
AGGGTTTCGTGCAGCATCCCGCGCTCATCGTTCAGCTTGAATTGCGCGATGCAGTTCTCGCAGACCTGACGGATTTCAAG
GTTCTCGCGGACCTGGACGTGGATGAGACGATCCATAGCTGCGAATGGCGCGACATGTTGGACAAAGGCGCGGTACCGCA
AACGCACCAGTTGCGCACCGCGCTTCTGGCGCGTGATTATCACGGGGTGATCTACCCCTCCTTTATGTCTCCCGGCGGCA
CCTGCGTCGCGCTCTGGCGTTGGAACGGTGCGAAAGAGCCGCGCTTGGATGTCATTGATCCCGAGGGCCGGCTGCCGAAA
TCACCGGCCTCGTGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13145.26 Da
• Isoelectric Point: 10.0414

>AT22254 WP_012061471.1 NC_009620:1026227-1026598 [Sinorhizobium medicae WSM419]
MMAVEFQIAAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSRVVRILAMASEMAGDESR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT22254 NC_009620:1026227-1026598 [Sinorhizobium medicae WSM419]
ATGATGGCCGTTGAGTTTCAGATAGCCGCTGCGCGTTTCGGGGACGATCACTCCCCATTTCTTTCGGCTCGCCTTGTCGC
CGATCGGCTGGGCGTCACGCTTGCCGAACTCGCAAAGCTGATCGGCGTTGCCCGGAATACGCTGACGGCGAAATCCGGCG
CCCGAAAGGTCGATAGTGCTTTAAGCAGGGTGGTGCGCATTCTGGCGATGGCTTCCGAAATGGCGGGCGACGAGAGCCGC
GCCGTCATTTGGTTCAAGCATCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTCGTCGGAGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCCGTTCGCGCCGGCGTCTACGCCTGA

Structures

Toxin

• AlphaFold DB: A0A508X590

Antitoxin

• AlphaFold DB: A0A508X7R7