Detailed information of TA system TA222529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 124828..125384
• Replicon: plasmid pSM-1
• Accession: NZ_CP086107
• Organism: Skermanella mucosa strain KEMB 2255-438

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JL100_RS30515
• Protein ID: WP_228421669.1
• Coordinates: 124828..125163 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JL100_RS30520
• Protein ID: WP_202683089.1
• Coordinates: 125160..125384 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL100_RS30490 (JL100_030570)	120672..121277	+	606	WP_202683084.1	TRAP transporter small permease	-
JL100_RS30495 (JL100_030575)	121274..122566	+	1293	WP_202683085.1	TRAP transporter large permease subunit	-
JL100_RS30500 (JL100_030580)	122585..122974	+	390	WP_202683086.1	cupin domain-containing protein	-
JL100_RS30505 (JL100_030585)	123013..124104	+	1092	WP_202683087.1	LacI family DNA-binding transcriptional regulator	-
JL100_RS30510 (JL100_030590)	124361..124780	+	420	Protein_143	IS5 family transposase	-
JL100_RS30515 (JL100_030595)	124828..125163	-	336	WP_228421669.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JL100_RS30520 (JL100_030600)	125160..125384	-	225	WP_202683089.1	antitoxin MazE family protein	Antitoxin
JL100_RS30525 (JL100_030605)	125705..126432	-	728	Protein_146	IS256 family transposase	-
JL100_RS30530 (JL100_030610)	126583..127041	+	459	WP_202683090.1	DUF1857 family protein	-
JL100_RS30535 (JL100_030615)	127259..128173	-	915	WP_202683091.1	CAP domain-containing protein	-
JL100_RS30540 (JL100_030620)	128517..128777	+	261	WP_228421628.1	hypothetical protein	-
JL100_RS30545 (JL100_030625)	128836..129051	-	216	Protein_150	TetR/AcrR family transcriptional regulator	-
JL100_RS30550 (JL100_030630)	129141..129545	-	405	WP_202683094.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..668738	668738
	-	flank	IS/Tn	-	-	126310..126432	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (6-68)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12004.87 Da
• Isoelectric Point: 10.1884

>T222529 WP_228421669.1 NZ_CP086107:c125163-124828 [Skermanella mucosa]
VRRGDLVTIAARIGGDYDGKPRPAVIIQSDDFPETDSVTVSLLTSIGIDAPLLRLRIDPAPANNLRQTSWVMIDKIATVR
RRNIGAVFGRLSDAELVQVNRALPVFLGIAG

Nucleotide

• Length: 336 bp

>T222529 NZ_CP117959:4642302-4642676 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8393.34 Da
• Isoelectric Point: 5.6624

>AT222529 WP_202683089.1 NZ_CP086107:c125384-125160 [Skermanella mucosa]
MPSTVRHRVQTHRAQLRAQGLRPIQIWVPDVRSPTFKEEARRQSRAVAAAPDEADTMDFLEDIQDLGDDAGDRP

Nucleotide

• Length: 225 bp

>AT222529 NZ_CP117959:4641844-4642212 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA