Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2138986..2140572 Replicon chromosome
Accession NZ_CP086104
Organism Ralstonia sp. RS650

Toxin (Protein)


Gene name hipA Uniprot ID A0A454TLL9
Locus tag LGV82_RS09860 Protein ID WP_058908490.1
Coordinates 2139235..2140572 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag LGV82_RS09855 Protein ID WP_028853251.1
Coordinates 2138986..2139231 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV82_RS09825 (LGV82_09800) 2135049..2135975 - 927 WP_265069412.1 muramoyltetrapeptide carboxypeptidase -
LGV82_RS09830 (LGV82_09805) 2135972..2136523 - 552 WP_011001400.1 tRNA adenosine(34) deaminase TadA -
LGV82_RS09835 (LGV82_09810) 2136543..2137019 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
LGV82_RS09840 (LGV82_09815) 2137087..2137725 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
LGV82_RS09845 (LGV82_09820) 2137897..2138016 + 120 Protein_1931 2-hydroxychromene-2-carboxylate isomerase -
LGV82_RS09850 (LGV82_09825) 2138043..2138882 + 840 WP_011001397.1 oxaloacetate decarboxylase -
LGV82_RS09855 (LGV82_09830) 2138986..2139231 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
LGV82_RS09860 (LGV82_09835) 2139235..2140572 + 1338 WP_058908490.1 type II toxin-antitoxin system HipA family toxin Toxin
LGV82_RS09865 (LGV82_09840) 2140569..2141771 + 1203 WP_011001394.1 MFS transporter -
LGV82_RS09870 (LGV82_09845) 2141997..2142725 + 729 WP_118872253.1 DsbA family protein -
LGV82_RS09875 (LGV82_09850) 2142738..2143334 - 597 WP_011001392.1 RNA 2',3'-cyclic phosphodiesterase -
LGV82_RS09880 (LGV82_09855) 2143482..2144180 - 699 WP_058908491.1 protocatechuate 3,4-dioxygenase subunit alpha -
LGV82_RS09885 (LGV82_09860) 2144177..2144917 - 741 WP_058908492.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48680.74 Da        Isoelectric Point: 6.8816

>T222519 WP_058908490.1 NZ_CP086104:2139235-2140572 [Ralstonia sp. RS650]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVA
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T222519 NZ_CP117959:c2012624-2012370 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT222519 WP_028853251.1 NZ_CP086104:2138986-2139231 [Ralstonia sp. RS650]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT222519 NZ_CP117959:c2012872-2012621 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454TLL9

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References