Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 505580..506213 Replicon chromosome
Accession NZ_CP086104
Organism Ralstonia sp. RS650

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag LGV82_RS02290 Protein ID WP_016725172.1
Coordinates 505800..506213 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag LGV82_RS02285 Protein ID WP_011002949.1
Coordinates 505580..505813 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV82_RS02270 (LGV82_02265) 501169..502463 - 1295 Protein_451 MFS transporter -
LGV82_RS02275 (LGV82_02270) 502471..503703 - 1233 WP_247394541.1 AGE family epimerase/isomerase -
LGV82_RS02280 (LGV82_02275) 503916..505523 + 1608 WP_247394543.1 glucan biosynthesis protein -
LGV82_RS02285 (LGV82_02280) 505580..505813 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LGV82_RS02290 (LGV82_02285) 505800..506213 + 414 WP_016725172.1 PIN domain-containing protein Toxin
LGV82_RS02295 (LGV82_02290) 506188..506814 - 627 WP_104072978.1 DUF1415 domain-containing protein -
LGV82_RS02300 (LGV82_02295) 507005..507376 + 372 WP_104072977.1 hypothetical protein -
LGV82_RS02305 (LGV82_02300) 507437..508933 - 1497 WP_104072976.1 glycerol kinase GlpK -
LGV82_RS02310 (LGV82_02305) 509095..510831 - 1737 WP_265069532.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T222518 WP_016725172.1 NZ_CP086104:505800-506213 [Ralstonia sp. RS650]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T222518 NZ_CP117959:1995023-1995397 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT222518 WP_011002949.1 NZ_CP086104:505580-505813 [Ralstonia sp. RS650]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT222518 NZ_CP117959:1994565-1994933 [Escherichia coli]
GTGTCAGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References