Detailed information of TA system TA222517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5884496..5885060
• Replicon: chromosome
• Accession: NZ_CP086102
• Organism: Streptomyces anulatus strain YINM00001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LK895_RS25235
• Protein ID: WP_228182780.1
• Coordinates: 5884713..5885060 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LK895_RS25230
• Protein ID: WP_228182779.1
• Coordinates: 5884496..5884726 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK895_RS25215	5880505..5881041	-	537	WP_047176630.1	hypothetical protein	-
LK895_RS25220	5881288..5881749	+	462	WP_228182777.1	hypothetical protein	-
LK895_RS25225	5881822..5884392	-	2571	WP_228182778.1	aminopeptidase N	-
LK895_RS25230	5884496..5884726	+	231	WP_228182779.1	ribbon-helix-helix domain-containing protein	Antitoxin
LK895_RS25235	5884713..5885060	+	348	WP_228182780.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LK895_RS25240	5885132..5886160	-	1029	WP_228182781.1	aspartate-semialdehyde dehydrogenase	-
LK895_RS25245	5886451..5889759	-	3309	WP_179233811.1	S8 family serine peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12736.56 Da
• Isoelectric Point: 10.7140

>T222517 WP_228182780.1 NZ_CP086102:5884713-5885060 [Streptomyces anulatus]
MRRGDIYLVDYEPVRGSEANKARPSIIVSNDGANSAVDRAGRGVVTVVPLTSNTSRLYPFQVLLQAGDSGLAKDSKVQCE
QVRAMAQERFVRQIGRVPRQRMTEIDAALRRHLAL

Nucleotide

• Length: 348 bp

>T222517 NZ_CP117959:c1805264-1805161 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8385.14 Da
• Isoelectric Point: 3.8382

>AT222517 WP_228182779.1 NZ_CP086102:5884496-5884726 [Streptomyces anulatus]
MKISVSLPQEDVAFVDEYATKTEADSRSAVIHAAIELLRASNLEADYVAAFAEWDESEDAAFWDKFSGDGIVDEAR

Nucleotide

• Length: 231 bp

>AT222517 NZ_CP117959:1805123-1805394 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT