Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1036528..1037148 Replicon chromosome
Accession NZ_CP086101
Organism Enterobacter ludwigii strain 994590

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag LL022_RS04915 Protein ID WP_014168902.1
Coordinates 1036528..1036746 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag LL022_RS04920 Protein ID WP_020885187.1
Coordinates 1036774..1037148 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL022_RS04885 (LL022_04895) 1032539..1032799 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
LL022_RS04890 (LL022_04900) 1032802..1032942 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LL022_RS04895 (LL022_04905) 1032939..1033649 - 711 WP_014168897.1 GNAT family protein -
LL022_RS04900 (LL022_04910) 1033750..1035210 + 1461 WP_228175955.1 PLP-dependent aminotransferase family protein -
LL022_RS04905 (LL022_04915) 1035182..1035649 - 468 WP_020885189.1 YlaC family protein -
LL022_RS04910 (LL022_04920) 1035767..1036318 - 552 WP_020885188.1 maltose O-acetyltransferase -
LL022_RS04915 (LL022_04925) 1036528..1036746 - 219 WP_014168902.1 HHA domain-containing protein Toxin
LL022_RS04920 (LL022_04930) 1036774..1037148 - 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
LL022_RS04925 (LL022_04935) 1037660..1040806 - 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit AcrB -
LL022_RS04930 (LL022_04940) 1040829..1042022 - 1194 WP_020885186.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T222505 WP_014168902.1 NZ_CP086101:c1036746-1036528 [Enterobacter ludwigii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

>T222505 NZ_CP117958:c36402-36127 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT222505 WP_020885187.1 NZ_CP086101:c1037148-1036774 [Enterobacter ludwigii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp

>AT222505 NZ_CP117958:c36686-36402 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGCGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References