Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 27188..27881 Replicon plasmid pCCBH28525_KPC
Accession NZ_CP086065
Organism Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag KK481_RS33290 Protein ID WP_003151133.1
Coordinates 27188..27565 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag KK481_RS33295 Protein ID WP_001172026.1
Coordinates 27546..27881 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KK481_RS33275 (KK481_033310) 22363..23214 - 852 Protein_28 IS1182-like element ISKpn6 family transposase -
KK481_RS33280 (KK481_033315) 23381..26410 - 3030 WP_010799689.1 Tn3 family transposase -
KK481_RS33285 (KK481_033320) 26394..26996 - 603 WP_010465829.1 recombinase family protein -
KK481_RS33290 (KK481_033325) 27188..27565 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KK481_RS33295 (KK481_033330) 27546..27881 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
KK481_RS33300 (KK481_033335) 27896..28231 + 336 WP_000741275.1 hypothetical protein -
KK481_RS33305 (KK481_033340) 28255..28581 + 327 WP_000091614.1 hypothetical protein -
KK481_RS33310 (KK481_033345) 28578..28958 + 381 WP_001054412.1 hypothetical protein -
KK481_RS33315 (KK481_033350) 29115..30396 + 1282 Protein_36 HD-GYP domain-containing protein -
KK481_RS33320 (KK481_033355) 30677..30916 - 240 WP_182325326.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..60312 60312
- flank IS/Tn blaKPC-2 - 32573..34540 1967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T222476 WP_003151133.1 NZ_CP086065:27188-27565 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T222476 NZ_CP117874:3378953-3379273 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCGCCTATAGCCGTCTGGCAACAACTTCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTCACGCTGAACGATACGCCGCTCTGTGAGGAAAATGTGATTCAGGAGCATATCG
ATGCTGGCGTCACGTTGGTCAATACCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTCACAGCCGTTGATGTTCTCCGTGCCAGACGCGCTACCGGCTTGCTCAAAGCATA
A

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT222476 WP_001172026.1 NZ_CP086065:27546-27881 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT222476 NZ_CP117874:3378548-3378883 [Pectobacterium carotovorum subsp. carotovorum]
ATGCCATCATCGGATACCCCGGAATGGGGGCTAAAGTGTGCTGTCACGCCGCACTTCGGAGCCAGGCTGGTTCAGGAAGG
TCACCGTCTGCATTATCTGGCAGACCGTGCCAGCATTGTCGGTACGTTCAGCAAAACAGTAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCAGTTGGAGCAGAAGCTACGCACGGGTGAACTGAATCCCCGCCAGCAGGGTTGTGTCACG
TTGCATTGCAACGAATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGTTATTTATCCCTCTTC
TGCGGCAGCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References