Detailed information of TA system TA222475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 8219..8817
• Replicon: plasmid pCCBH28525_KPC
• Accession: NZ_CP086065
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6H1Q9F7
• Locus tag: KK481_RS33185
• Protein ID: WP_028692294.1
• Coordinates: 8515..8817 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KK481_RS33180
• Protein ID: WP_028692295.1
• Coordinates: 8219..8518 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS33160 (KK481_033195)	3594..4873	-	1280	Protein_5	Y-family DNA polymerase	-
KK481_RS33165 (KK481_033200)	4863..5291	-	429	WP_028691923.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KK481_RS33170 (KK481_033205)	7360..7683	-	324	WP_028692297.1	hypothetical protein	-
KK481_RS33175 (KK481_033210)	7978..8199	-	222	WP_028692296.1	hypothetical protein	-
KK481_RS33180 (KK481_033215)	8219..8518	-	300	WP_028692295.1	putative addiction module antidote protein	Antitoxin
KK481_RS33185 (KK481_033220)	8515..8817	-	303	WP_028692294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KK481_RS33190 (KK481_033225)	9020..9799	+	780	WP_028692293.1	DUF4942 domain-containing protein	-
KK481_RS33195 (KK481_033230)	9952..10320	+	369	WP_028692292.1	hypothetical protein	-
KK481_RS33200 (KK481_033235)	10347..10664	+	318	WP_028692291.1	hypothetical protein	-
KK481_RS33205 (KK481_033240)	11002..12093	+	1092	WP_051555741.1	hypothetical protein	-
KK481_RS33210 (KK481_033245)	12239..12481	+	243	WP_028692289.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..60312	60312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11277.85 Da
• Isoelectric Point: 10.3903

>T222475 WP_028692294.1 NZ_CP086065:c8817-8515 [Pseudomonas aeruginosa]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKSLASQWSDDE

Nucleotide

• Length: 303 bp

>T222475 NZ_CP117874:c3023982-3023662 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAACCATCACCTACAATCCCGTCACGGGAGGATAACCCCTGTGCGTCACCTATAGCTGTCTGGCAGCAGCTTCTGAC
CTACCTGCTGGAAAAGCACTACGGTCTCACGTTGAACGACACACCGTTTTGCGAGAAAAGTGAGATTCAGGAACATATCG
ATGCTGGCATCACATTGGTCAATGCCGTCAATTTTTTGGTGGAAAAATACGAACTGGTGCGTATCGATCACAACGGTTTT
AACTGGCAGGAGCAATCACCGTTTCTCACCGCTGTTGATGTTCTCCGTGCCAGACGCGCTACTGGCTTGCTCAAGGCATA
A

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10825.27 Da
• Isoelectric Point: 5.1084

>AT222475 WP_028692295.1 NZ_CP086065:c8518-8219 [Pseudomonas aeruginosa]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPAH

Nucleotide

• Length: 300 bp

>AT222475 NZ_CP117874:c3024387-3024052 [Pectobacterium carotovorum subsp. carotovorum]
ATGCCATCATCGGATACCCCTGAATGGGGACTAAAATGTGCCATCACTCCGCGATTCGGAGCTAGGCTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGCAGATCGGGCCAGCGTTGTCGGTACGTTCAGCAAAACAGGAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCAGTTGGAGCAGAAGCTACGCACGGGTGAACTGAATCCCCGCCAGCAGGGTTGTGTCACG
TTGCATTGCAACGAATGGGCCTGTGAAGCTGATACGCTGGGCAGTGTTGGTTACGTGTATATCGTTATTTATCCAACTTC
TGCTGCAACACTGTAA

Structures

Toxin

• AlphaFold DB: A0A6H1Q9F7