Detailed information of TA system TA222473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2871398..2872619
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KK481_RS14045
• Protein ID: WP_003114949.1
• Coordinates: 2871398..2871976 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KK481_RS14050
• Protein ID: WP_003088112.1
• Coordinates: 2872065..2872619 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS14025 (KK481_014040)	2866432..2866800	+	369	WP_003127781.1	Mor transcription activator family protein	-
KK481_RS14030 (KK481_014045)	2866873..2867952	+	1080	WP_228927827.1	TolC family protein	-
KK481_RS14035 (KK481_014050)	2867942..2870113	+	2172	WP_019726105.1	type I secretion system permease/ATPase	-
KK481_RS14040 (KK481_014055)	2870103..2871290	+	1188	WP_019726106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KK481_RS14045 (KK481_014060)	2871398..2871976	-	579	WP_003114949.1	HD domain-containing protein	Toxin
KK481_RS14050 (KK481_014065)	2872065..2872619	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
KK481_RS14055 (KK481_014070)	2872777..2874972	-	2196	WP_003450155.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
KK481_RS14060 (KK481_014075)	2874977..2875438	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
KK481_RS14065 (KK481_014080)	2875769..2876092	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
KK481_RS14070 (KK481_014085)	2876105..2876503	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
KK481_RS14075 (KK481_014090)	2876500..2877051	-	552	WP_003132721.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	lasA	2814788..2880102	65314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T222473 WP_003114949.1 NZ_CP086064:c2871976-2871398 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T222473 NZ_CP117874:1708759-1709112 [Pectobacterium carotovorum subsp. carotovorum]
GTGTGGAGAATTGAAACGACGGACGTGTTTGATCGCTGGTTCAGCGCCTTAAACGATAGGGAGCGAGCTTGTGTGCTTGC
AGCACTTATCGTGTTGCGGGAAAAAGGTCCAATGTTATCTAGACCTTATGCCGATACGGTTAAAGGTTCTCGCTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGAACGCCGATACGGGCATTTTTTGCCTTCGACCCATATCGTACAGGGATC
CTGCTCTGCGCGGGCAATAAGTCCGGCAATGAAAAGCGCTTTTACGATGAGATGCTCCCGTTTGCTGAGCGGGAGTTAAC
AAATTATCTGAAAAAATCAGAGAAGAGGGAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT222473 WP_003088112.1 NZ_CP086064:c2872619-2872065 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT222473 NZ_CP117874:1709117-1709419 [Pectobacterium carotovorum subsp. carotovorum]
ATGGGCAGAACGCTGGAACAGCTTCTTGCGGATGAAAAGCCAGAGGTCGTGGTCGATGCTCACGCTATAGCGACAGAGAT
GCTGCTCAACATTCATCTCGCCGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGAAATGGCGCAGATGCTTGGGTTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGTCGCGATCTGAAACTTTCCACGCTTAAACGCTATGTCGAAGCGACT
GGCAGTAAGCTACGTCTTGATGTTGAACTGCCGGACGGCTCGCATTACGAATTTATGCTATAG