Detailed information of TA system TA222472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1808730..1809988
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KK481_RS08840
• Protein ID: WP_019725936.1
• Coordinates: 1809233..1809988 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KK481_RS08835
• Protein ID: WP_003086329.1
• Coordinates: 1808730..1809098 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS08815 (KK481_008825)	1804893..1805168	+	276	WP_010792073.1	zinc ribbon domain-containing protein	-
KK481_RS08820 (KK481_008830)	1805171..1805647	-	477	WP_019725934.1	hypothetical protein	-
KK481_RS08825 (KK481_008835)	1805726..1807315	+	1590	WP_019725935.1	aldehyde dehydrogenase family protein	-
KK481_RS08830 (KK481_008840)	1807371..1808657	+	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KK481_RS08835 (KK481_008845)	1808730..1809098	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KK481_RS08840 (KK481_008850)	1809233..1809988	+	756	WP_019725936.1	RES family NAD+ phosphorylase	Toxin
KK481_RS08845 (KK481_008855)	1810757..1812118	+	1362	WP_003081943.1	DNA recombination protein RmuC	-
KK481_RS08850 (KK481_008860)	1812273..1814816	-	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28192.33 Da
• Isoelectric Point: 8.0554

>T222472 WP_019725936.1 NZ_CP086064:1809233-1809988 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSGHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T222472 NZ_CP117874:c1267300-1267097 [Pectobacterium carotovorum subsp. carotovorum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCTGAACTGACAATGAACAAAC
TCTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT222472 WP_003086329.1 NZ_CP086064:1808730-1809098 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT222472 NZ_CP117874:c1267722-1267354 [Pectobacterium carotovorum subsp. carotovorum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
GACGTTAGGCGATAGCAGTCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGCATTAATGATGCTGAACTACGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7