Detailed information of TA system TA222470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1451457..1452065
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: KK481_RS07005
• Protein ID: WP_016263850.1
• Coordinates: 1451718..1452065 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: KK481_RS07000
• Protein ID: WP_003114155.1
• Coordinates: 1451457..1451708 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS06980 (KK481_006985)	1447095..1447445	+	351	WP_019725830.1	DUF2523 family protein	-
KK481_RS06985 (KK481_006990)	1447447..1448709	+	1263	WP_019725831.1	zonular occludens toxin domain-containing protein	-
KK481_RS06990 (KK481_006995)	1448968..1450260	+	1293	WP_003115206.1	hypothetical protein	-
KK481_RS06995 (KK481_007000)	1450260..1451243	+	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
KK481_RS07000 (KK481_007005)	1451457..1451708	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KK481_RS07005 (KK481_007010)	1451718..1452065	+	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KK481_RS07015 (KK481_007020)	1452392..1453294	-	903	WP_003123042.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
KK481_RS07020 (KK481_007025)	1453746..1454483	-	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
KK481_RS07025 (KK481_007030)	1454563..1455606	-	1044	WP_003098363.1	L,D-transpeptidase	-
KK481_RS07030 (KK481_007035)	1455742..1456434	-	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
KK481_RS07035 (KK481_007040)	1456434..1456706	-	273	WP_019725834.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1437531..1452065	14534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T222470 WP_016263850.1 NZ_CP086064:1451718-1452065 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T222470 NZ_CP117874:566952-567440 [Pectobacterium carotovorum subsp. carotovorum]
ATGATTTCCGCCCCTGAGCCACTCCACGCCGAACATGTACTATCTTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GTTGAAACAACGGGCGATGAAAAATCAGGTCACTGGAGCATCGCGAACTTTCGTCAGTTGCGATGGCGCTAAAGTGCTAG
CGTATTACTCATTGGCCTCCAGTGCCGTAGCAACGAATGCCGCCCCCGGACGTTTTCGCCGCAATATGCCCGATCCGATC
CCGGTTGTGGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAAGGTGTTGGTCGGGCGCTGGTGCGTGATGC
GGGGCTGCGGGTGATTCAGGTGGCAGATACTATTGGCATTCGAGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGGG
AATTTTACCTGCGTGTGGGATTTGAGCCTTCGCCGATGGATCCGATGATGTTGATGGTGACGCTGGGGGATTTGGTAGAT
AGTCTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT222470 WP_003114155.1 NZ_CP086064:1451457-1451708 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT222470 NZ_CP117874:566662-566955 [Pectobacterium carotovorum subsp. carotovorum]
ATGCCAGCGACAAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCTGAACGCGATCTAATCGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTCGAAGCTGCCCGTGCTGCCGCAGAGGAAGCATTGA
TCGATCAGCGCATCATTATGGCAGATCCAGACGCTTATCAAGAATTTCTTACACGATTGGATCAAGCTCCTGCACCAAAT
GCGGCGCTGCGTAAAACCATGCAAACGCCAGCACCGTGGGAACTGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355