Detailed information of TA system TA222467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147963..148468
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: KK481_RS00665
• Protein ID: WP_003083773.1
• Coordinates: 147963..148244 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: KK481_RS00670
• Protein ID: WP_003083775.1
• Coordinates: 148241..148468 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS00640 (KK481_000640)	143214..144563	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
KK481_RS00645 (KK481_000645)	144612..145298	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
KK481_RS00650 (KK481_000650)	145399..146133	+	735	WP_003117208.1	GntR family transcriptional regulator	-
KK481_RS00655 (KK481_000655)	146313..146723	+	411	WP_003101225.1	aegerolysin family protein	-
KK481_RS00660 (KK481_000660)	146755..147663	-	909	WP_003083769.1	LysR family transcriptional regulator	-
KK481_RS00665 (KK481_000665)	147963..148244	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
KK481_RS00670 (KK481_000670)	148241..148468	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
KK481_RS00675 (KK481_000675)	148644..149264	-	621	WP_009314940.1	hypothetical protein	-
KK481_RS00680 (KK481_000680)	149365..149865	+	501	WP_003112629.1	LEA type 2 family protein	-
KK481_RS00685 (KK481_000685)	149938..150279	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
KK481_RS00690 (KK481_000690)	150361..151788	-	1428	WP_003083784.1	GABA permease	-
KK481_RS00695 (KK481_000695)	151957..153450	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T222467 WP_003083773.1 NZ_CP086064:c148244-147963 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T222467 NZ_CP117874:409876-410187 [Pectobacterium carotovorum subsp. carotovorum]
ATGCATGTTATTTCGCGGGCACCTTTCGACACTGCAACCACTCAGTTTCCGAATCAGGCGGCAGCACTTGCTGATCTATA
TCGGGTTATCAAACGCGAAATGTATGCAACGCCGGACGATATGAAAAAACGCTTCCCTAGCCTGGATAGGATGAAATATC
GGGAAAAGTGGTGGGTTATTGATATTGGCGGTGGGCATCTTCGAGTGATGTTTTTTGCTGATTTTGAGCGGGGGAAAATC
TTCATCAAGCACATCACATCCCATGCAGAGTATGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT222467 WP_003083775.1 NZ_CP086064:c148468-148241 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT222467 NZ_CP117874:410184-410600 [Pectobacterium carotovorum subsp. carotovorum]
ATGATGTACGCAGACGCCATCAAGGCAGCTAACAACCTGACGAGTATCGTACCATTCCTCGGAGGCAGCACCTCTCGTAA
GGATTATGAGGATGCGCTGAAACTCACTGAATACCTGATCGAGAGTGAGCCTGATAGTCCGCTAATCGATATGCTGACCG
CCAAAATTGACAAATACGAAGATGATGCGCCGGAGTTTGCTGCCTTCAACGAACGTATTAAAGCACTCCCAGCCGGTATC
GCTTTACTGCGCGTCCTCATGGATCAGTACCAGCTAACGCAGAGTGATTTTGAAGCTGAGATCGGTAAAAAGTCACTGGT
ATCCCGCATTCTAAATGGTGAGCGCACTCTAACGCTTGATCACATGAGAGCGCTTGCTAAACGATTCGATATTCCTGTGA
GTGCTTTTGTTGATTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9