Detailed information of TA system TA222451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 3055778..3056772
• Replicon: chromosome
• Accession: NZ_CP086060
• Organism: Paracidovorax citrulli strain HPP21-9-4B

Toxin (Protein)

• Gene name: avrRxo1
• Uniprot ID: -
• Locus tag: LKW30_RS14125
• Protein ID: WP_269149363.1
• Coordinates: 3056089..3056772 (-)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: arc1
• Uniprot ID: A1TRP1
• Locus tag: LKW30_RS14120
• Protein ID: WP_011796139.1
• Coordinates: 3055778..3056074 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW30_RS14110	3054073..3054870	+	798	WP_228193814.1	BMA_0021/BMA_0022 family TOMM bacteriocin	-
LKW30_RS14115	3054880..3055734	+	855	WP_228193813.1	class I SAM-dependent methyltransferase	-
LKW30_RS14120	3055778..3056074	-	297	WP_011796139.1	hypothetical protein	Antitoxin
LKW30_RS14125	3056089..3056772	-	684	WP_269149363.1	XopAJ/AvrRxo1 family type III secretion system effector zeta toxin	Toxin
LKW30_RS14130	3057109..3057378	+	270	WP_228193812.1	hypothetical protein	-
LKW30_RS14135	3057610..3059796	+	2187	WP_228193811.1	TOMM precursor leader peptide-binding protein	-
LKW30_RS14140	3059930..3060784	+	855	WP_233249072.1	FHA domain-containing protein	-
LKW30_RS22230	3060950..3061078	+	129	WP_258868820.1	hypothetical protein	-
LKW30_RS14145	3061100..3061438	+	339	WP_011796135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 24279.43 Da
• Isoelectric Point: 5.1194

>T222451 WP_269149363.1 NZ_CP086060:c3056772-3056089 [Paracidovorax citrulli]
IFKRSLAETPEGAKLTSAQVHAESCILADRLEDELRLQKTASGAAISMLVDKRLAGAHEIDAYIKLAQETGRKIELCDID
APLEQSLMGVLQRKPEGDAPRPPYVAVANGFSAVRGHRLDVIDKFLSNPTLGSYHLFGTTGNGSKAMVASVVNGELSIHD
PQLYEAITDPQGARAGDSGDQIIDAALIDRLTKGIDDPARAMDARTALERYAGMTWSDALAAHSSLT

Nucleotide

• Length: 684 bp

>T222451 NZ_CP117858:101126-101455 [Komagataeibacter oboediens]
ATGACTGAATACGCGATCGAGTTCGATGATCGTGCACTTCGGGAATGGGACGGGCTGGACGGAAGCATTCGCAGGAAATT
CGAGAAGAAGCTCGAAAAACTGGTTCTGACCCCGCATTCTCCCGGCAATGAACTGCACGGCGATCTCGCCGGATTCTACA
AAATCAAGCTTCGCCAGGACGACTATCAGGTCGTTGAACAGAGGATCGTTATTTTTGTCATCGCGATGGGGCGCCGCGAG
GATGATGAAATCTACACGGCGGCAACAGGGCGCGTTCCGGAAACGCCAGTCACTGCTGCAAACCCTGGTAGACTTGGAAC
TGACCGATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11106.62 Da
• Isoelectric Point: 6.9795

>AT222451 WP_011796139.1 NZ_CP086060:c3056074-3055778 [Paracidovorax citrulli]
MTGLKGADALDFHKKLKERNAALRNAELNAAKELADKNGKEPFDLVKLERLCDTSQAGRLVEGTERQAMYEYMYYVEYKN
VGTLQDFARVVTTLASWS

Nucleotide

• Length: 297 bp

>AT222451 NZ_CP117858:100870-101133 [Komagataeibacter oboediens]
ATGTCCGCCGTGACATTCCGCGTCGATGACGCCCTCAAATCAGCTGCCGTTGCAAAACTCTCCGCGCACGGCCTTTCTCT
CTCCGATGTCCTGCGCGATACGTTGGCCTATATCGCCGAGACCGGCCAGCCGCCGGTAAAGCGGCGGCTTGTGACCGACG
AAGATGCTAGGCTGATCGAAATCGTCCGCGAACGCCTCGCCGATCCCGCCCCCCGCCACCGCATGACGCTGGCGGAGCTC
AAGGCCCGCCATCCCGATGACTGA

Structures

Antitoxin

• AlphaFold DB: A1TRP1