Detailed information of TA system TA222450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 2657473..2658467
• Replicon: chromosome
• Accession: NZ_CP086023
• Organism: Paracidovorax citrulli strain HPP21-3-3B

Toxin (Protein)

• Gene name: avrRxo1
• Uniprot ID: -
• Locus tag: LKW27_RS11875
• Protein ID: WP_269149363.1
• Coordinates: 2657473..2658156 (+)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: arc1
• Uniprot ID: A1TRP1
• Locus tag: LKW27_RS11880
• Protein ID: WP_011796139.1
• Coordinates: 2658171..2658467 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW27_RS11855 (LKW27_11880)	2652807..2653145	-	339	WP_011796135.1	hypothetical protein	-
LKW27_RS22230	2653167..2653295	-	129	WP_258868820.1	hypothetical protein	-
LKW27_RS11860 (LKW27_11885)	2653461..2654384	-	924	WP_228193810.1	FHA domain-containing protein	-
LKW27_RS11865 (LKW27_11890)	2654449..2656635	-	2187	WP_228193811.1	TOMM precursor leader peptide-binding protein	-
LKW27_RS11870 (LKW27_11895)	2656867..2657136	-	270	WP_228193812.1	hypothetical protein	-
LKW27_RS11875 (LKW27_11900)	2657473..2658156	+	684	WP_269149363.1	XopAJ/AvrRxo1 family type III secretion system effector zeta toxin	Toxin
LKW27_RS11880 (LKW27_11905)	2658171..2658467	+	297	WP_011796139.1	hypothetical protein	Antitoxin
LKW27_RS11885 (LKW27_11910)	2658511..2659365	-	855	WP_228193813.1	class I SAM-dependent methyltransferase	-
LKW27_RS11890 (LKW27_11915)	2659375..2660172	-	798	WP_228193814.1	BMA_0021/BMA_0022 family TOMM bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 24279.43 Da
• Isoelectric Point: 5.1194

>T222450 WP_269149363.1 NZ_CP086023:2657473-2658156 [Paracidovorax citrulli]
IFKRSLAETPEGAKLTSAQVHAESCILADRLEDELRLQKTASGAAISMLVDKRLAGAHEIDAYIKLAQETGRKIELCDID
APLEQSLMGVLQRKPEGDAPRPPYVAVANGFSAVRGHRLDVIDKFLSNPTLGSYHLFGTTGNGSKAMVASVVNGELSIHD
PQLYEAITDPQGARAGDSGDQIIDAALIDRLTKGIDDPARAMDARTALERYAGMTWSDALAAHSSLT

Nucleotide

• Length: 684 bp

>T222450 NZ_CP117857:c1293429-1292683 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCACCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGTCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11106.62 Da
• Isoelectric Point: 6.9795

>AT222450 WP_011796139.1 NZ_CP086023:2658171-2658467 [Paracidovorax citrulli]
MTGLKGADALDFHKKLKERNAALRNAELNAAKELADKNGKEPFDLVKLERLCDTSQAGRLVEGTERQAMYEYMYYVEYKN
VGTLQDFARVVTTLASWS

Nucleotide

• Length: 297 bp

>AT222450 NZ_CP117857:c1292682-1292512 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A1TRP1