Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2208567..2210153 Replicon chromosome
Accession NZ_CP086018
Organism Ralstonia sp. RS642

Toxin (Protein)


Gene name hipA Uniprot ID A0A454TLL9
Locus tag LGV80_RS10420 Protein ID WP_058908490.1
Coordinates 2208816..2210153 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag LGV80_RS10415 Protein ID WP_028853251.1
Coordinates 2208567..2208812 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV80_RS10385 (LGV80_10355) 2204630..2205556 - 927 WP_028860692.1 muramoyltetrapeptide carboxypeptidase -
LGV80_RS10390 (LGV80_10360) 2205553..2206104 - 552 WP_011001400.1 tRNA adenosine(34) deaminase TadA -
LGV80_RS10395 (LGV80_10365) 2206124..2206600 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
LGV80_RS10400 (LGV80_10370) 2206668..2207306 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
LGV80_RS10405 (LGV80_10375) 2207478..2207597 + 120 Protein_2043 2-hydroxychromene-2-carboxylate isomerase -
LGV80_RS10410 (LGV80_10380) 2207624..2208463 + 840 WP_011001397.1 oxaloacetate decarboxylase -
LGV80_RS10415 (LGV80_10385) 2208567..2208812 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
LGV80_RS10420 (LGV80_10390) 2208816..2210153 + 1338 WP_058908490.1 type II toxin-antitoxin system HipA family toxin Toxin
LGV80_RS10425 (LGV80_10395) 2210150..2211352 + 1203 WP_011001394.1 MFS transporter -
LGV80_RS10430 (LGV80_10400) 2211578..2212306 + 729 WP_011001393.1 DsbA family protein -
LGV80_RS10435 (LGV80_10405) 2212329..2212925 - 597 WP_011001392.1 RNA 2',3'-cyclic phosphodiesterase -
LGV80_RS10440 (LGV80_10410) 2213073..2213771 - 699 WP_058908491.1 protocatechuate 3,4-dioxygenase subunit alpha -
LGV80_RS10445 (LGV80_10415) 2213768..2214508 - 741 WP_058908492.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48680.74 Da        Isoelectric Point: 6.8816

>T222448 WP_058908490.1 NZ_CP086018:2208816-2210153 [Ralstonia sp. RS642]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVA
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T222448 NZ_CP117853:c4737085-4736828 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT222448 WP_028853251.1 NZ_CP086018:2208567-2208812 [Ralstonia sp. RS642]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT222448 NZ_CP117853:c4736817-4736266 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGTGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454TLL9

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References