Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 532997..533630 Replicon chromosome
Accession NZ_CP086018
Organism Ralstonia sp. RS642

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag LGV80_RS02635 Protein ID WP_016725172.1
Coordinates 533217..533630 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag LGV80_RS02630 Protein ID WP_011002949.1
Coordinates 532997..533230 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV80_RS02615 (LGV80_02620) 528585..529880 - 1296 WP_011002952.1 MFS transporter -
LGV80_RS02620 (LGV80_02625) 529888..531120 - 1233 WP_231679073.1 AGE family epimerase/isomerase -
LGV80_RS02625 (LGV80_02630) 531333..532940 + 1608 WP_247394543.1 glucan biosynthesis protein -
LGV80_RS02630 (LGV80_02635) 532997..533230 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LGV80_RS02635 (LGV80_02640) 533217..533630 + 414 WP_016725172.1 PIN domain-containing protein Toxin
LGV80_RS02640 (LGV80_02645) 533605..534231 - 627 WP_104072978.1 DUF1415 domain-containing protein -
LGV80_RS02645 (LGV80_02650) 534422..534793 + 372 WP_104072977.1 hypothetical protein -
LGV80_RS02650 (LGV80_02655) 534854..536350 - 1497 WP_104072976.1 glycerol kinase GlpK -
LGV80_RS02655 (LGV80_02660) 536512..538248 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T222447 WP_016725172.1 NZ_CP086018:533217-533630 [Ralstonia sp. RS642]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T222447 NZ_CP117853:4481152-4481370 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT222447 WP_011002949.1 NZ_CP086018:532997-533230 [Ralstonia sp. RS642]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT222447 NZ_CP117853:4480751-4481125 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTACGCCGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References