Detailed information of TA system TA222444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2350315..2351572
• Replicon: chromosome
• Accession: NZ_CP086016
• Organism: Pseudomonas aeruginosa isolate KB-PA_3

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A9JV85
• Locus tag: LKM46_RS11500
• Protein ID: WP_033969419.1
• Coordinates: 2350817..2351572 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LKM46_RS11495
• Protein ID: WP_042167402.1
• Coordinates: 2350315..2350683 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKM46_RS11475 (LKM46_11475)	2346481..2346756	+	276	WP_177344468.1	zinc ribbon domain-containing protein	-
LKM46_RS11480 (LKM46_11480)	2346759..2347235	-	477	WP_033976822.1	hypothetical protein	-
LKM46_RS11485 (LKM46_11485)	2347313..2348902	+	1590	WP_079387357.1	aldehyde dehydrogenase family protein	-
LKM46_RS11490 (LKM46_11490)	2348956..2350242	+	1287	WP_042167399.1	FAD-binding oxidoreductase	-
LKM46_RS11495 (LKM46_11495)	2350315..2350683	+	369	WP_042167402.1	DUF2384 domain-containing protein	Antitoxin
LKM46_RS11500 (LKM46_11500)	2350817..2351572	+	756	WP_033969419.1	RES family NAD+ phosphorylase	Toxin
LKM46_RS11505 (LKM46_11505)	2352344..2353705	+	1362	WP_003134034.1	DNA recombination protein RmuC	-
LKM46_RS11510 (LKM46_11510)	2353860..2356403	-	2544	WP_079387358.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28253.37 Da
• Isoelectric Point: 8.0554

>T222444 WP_033969419.1 NZ_CP086016:2350817-2351572 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQATPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVNGKLPHPA

Nucleotide

• Length: 756 bp

>T222444 NZ_CP117853:918102-918512 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAACTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACTCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13366.64 Da
• Isoelectric Point: 10.2737

>AT222444 WP_042167402.1 NZ_CP086016:2350315-2350683 [Pseudomonas aeruginosa]
MSALIKQRPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT222444 NZ_CP117853:917855-918121 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTCAGCGATGGCGACAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6A9JV85