Detailed information of TA system TA222443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1777953..1778561
• Replicon: chromosome
• Accession: NZ_CP086016
• Organism: Pseudomonas aeruginosa isolate KB-PA_3

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: LKM46_RS08470
• Protein ID: WP_042168819.1
• Coordinates: 1778214..1778561 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: LKM46_RS08465
• Protein ID: WP_003114155.1
• Coordinates: 1777953..1778204 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKM46_RS08445 (LKM46_08440)	1773626..1774150	+	525	WP_042168829.1	helix-turn-helix transcriptional regulator	-
LKM46_RS08450 (LKM46_08445)	1774147..1775415	+	1269	WP_042168826.1	HipA domain-containing protein	-
LKM46_RS08455 (LKM46_08450)	1775490..1776515	-	1026	WP_228240906.1	fimbrial protein	-
LKM46_RS08460 (LKM46_08455)	1777137..1777739	+	603	Protein_1670	integrase	-
LKM46_RS08465 (LKM46_08460)	1777953..1778204	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LKM46_RS08470 (LKM46_08465)	1778214..1778561	+	348	WP_042168819.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKM46_RS08475 (LKM46_08470)	1778838..1779479	-	642	WP_003158902.1	substrate-binding domain-containing protein	-
LKM46_RS08480 (LKM46_08475)	1779601..1779906	+	306	WP_003158903.1	helix-turn-helix transcriptional regulator	-
LKM46_RS08485 (LKM46_08480)	1780005..1782353	-	2349	WP_176031468.1	S8 family peptidase	-
LKM46_RS08490 (LKM46_08485)	1782493..1783524	-	1032	WP_003158906.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	catB7 / sul1 / blaOXA-10 / blaIMP-14	-	1678285..1896854	218569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12978.82 Da
• Isoelectric Point: 4.5511

>T222443 WP_042168819.1 NZ_CP086016:1778214-1778561 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGELAALQSVLSLLDEIEEKISLAPKGYPVSHQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T222443 NZ_CP117853:761895-762272 [Klebsiella michiganensis]
ATGCAAACACAACCTGTTCCCCCGACACAGGAGGCTTCACCACGCCCGTCACCAGTGGAGATCTGGCTCCGCCTTTTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGTTAAACGACACGCCATTTTGCAATGATGGTGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGCCCTGATACGCACCGATCGTCACGGTTTC
AGCGCAGAAACACAGTCCCCACTCATCAGCAGTATTGATATCCTTCGCGCCCGTAAGGCCTGTGGACTGATGGCACGCAA
TGGCTACAGACTGGTGACCGATATCACCACCGGCAAATACAGCGAGGTGACTCGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT222443 WP_003114155.1 NZ_CP086016:1777953-1778204 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT222443 NZ_CP117853:761486-761845 [Klebsiella michiganensis]
TTGTCGAATAAAATACCGCCTGTAAACCACAATATCGCCGAACCCTGGTGGGGGCTTAAACGCGATATCACACCCTGTTT
TGGCGCTCGCCTGGTGCAGGAGGGCAACCGGCTGCACTATCTCGCCGACCGCGCCAGTATCACCGGGCAGTTCAGCGACG
CGGATTTACGCCATCTGGACCAGGCATTTCCGCTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAAT
CCCCGCCATCAGCACGGCGTCACGCTCTACGCAAAAGGGCTGACCTGCAACGCGGATACACTCGGCAGTTGCGGATATGT
CTATATAGCGATTTACCCGACACCTGCAACAACAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355