Detailed information of TA system TA222440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 144494..144999
• Replicon: chromosome
• Accession: NZ_CP086016
• Organism: Pseudomonas aeruginosa isolate KB-PA_3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LKM46_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 144494..144775 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LKM46_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 144772..144999 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKM46_RS00635 (LKM46_00635)	139745..141094	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
LKM46_RS00640 (LKM46_00640)	141143..141829	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LKM46_RS00645 (LKM46_00645)	141930..142664	+	735	WP_003117208.1	GntR family transcriptional regulator	-
LKM46_RS00650 (LKM46_00650)	142844..143254	+	411	WP_003101225.1	aegerolysin family protein	-
LKM46_RS00655 (LKM46_00655)	143286..144194	-	909	WP_010793581.1	LysR family transcriptional regulator	-
LKM46_RS00660 (LKM46_00660)	144494..144775	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LKM46_RS00665 (LKM46_00665)	144772..144999	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LKM46_RS00670 (LKM46_00670)	145175..145795	-	621	WP_003101226.1	hypothetical protein	-
LKM46_RS00675 (LKM46_00675)	145896..146396	+	501	WP_003101228.1	LEA type 2 family protein	-
LKM46_RS00680 (LKM46_00680)	146469..146810	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LKM46_RS00685 (LKM46_00685)	146892..148319	-	1428	WP_003083784.1	GABA permease	-
LKM46_RS00690 (LKM46_00690)	148488..149981	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T222440 WP_003083773.1 NZ_CP086016:c144775-144494 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T222440 NZ_CP117852:4510386-4510631 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATCAACAGCAAAGCCTGCATGACCACCTGTTACTCACAAATCCCCAGCCAGCACCTTAAGGGTGACTGGCAGGAAGAAGC
GGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGA
GGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACGGG
GATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT222440 WP_003083775.1 NZ_CP086016:c144999-144772 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT222440 NZ_CP117852:c4510621-4510210 [Salmonella enterica subsp. enterica serovar Mbandaka]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGTGGTCATGCAGGCTTTGCTGTTGATGTGG
CGGACGCTGACGCCGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAG
CTCAGGGCGAGCGAGTATGGGCGCGACATCCTGGCACTGCGCTCCCCTCTCCCCGGCCGGTGATGAGCAGCCGGCTGCCG
GGGCAGTATTTT

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9