Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3122122..3122692 Replicon chromosome
Accession NC_009565
Organism Mycobacterium tuberculosis F11

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag TBFG_RS14835 Protein ID WP_003414166.1
Coordinates 3122122..3122478 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag TBFG_RS14840 Protein ID WP_003901465.1
Coordinates 3122462..3122692 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBFG_RS14815 3117574..3119262 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
TBFG_RS14820 3119266..3119592 - 327 WP_003414157.1 hypothetical protein -
TBFG_RS14825 3119765..3120352 + 588 WP_023641719.1 DUF3558 family protein -
TBFG_RS14830 3120371..3122020 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
TBFG_RS14835 3122122..3122478 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
TBFG_RS14840 3122462..3122692 - 231 WP_003901465.1 antitoxin MazE Antitoxin
TBFG_RS14845 3122735..3123778 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
TBFG_RS22195 3123969..3124244 + 276 WP_003911993.1 DUF1778 domain-containing protein -
TBFG_RS22200 3124420..3124674 - 255 WP_003917684.1 hypothetical protein -
TBFG_RS14860 3124822..3125226 + 405 WP_009938577.1 hypothetical protein -
TBFG_RS14865 3125223..3125414 + 192 WP_003414184.1 hypothetical protein -
TBFG_RS14870 3125478..3126767 + 1290 Protein_2906 transposase family protein -
TBFG_RS14875 3127001..3127258 + 258 WP_003899489.1 hypothetical protein -
TBFG_RS14880 3127363..3127674 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T22244 WP_003414166.1 NC_009565:c3122478-3122122 [Mycobacterium tuberculosis F11]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T22244 NC_009565:c3122478-3122122 [Mycobacterium tuberculosis F11]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT22244 WP_003901465.1 NC_009565:c3122692-3122462 [Mycobacterium tuberculosis F11]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT22244 NC_009565:c3122692-3122462 [Mycobacterium tuberculosis F11]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References