Detailed information of TA system TA222437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4720610..4721250
• Replicon: chromosome
• Accession: NZ_CP086010
• Organism: Pseudomonas aeruginosa isolate KB-PA_F19

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LLH04_RS22200
• Protein ID: WP_003134109.1
• Coordinates: 4720610..4721020 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: LLH04_RS22205
• Protein ID: WP_023132185.1
• Coordinates: 4721020..4721250 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLH04_RS22160 (LLH04_22175)	4716725..4717453	+	729	WP_057387876.1	TIGR03761 family integrating conjugative element protein	-
LLH04_RS22165 (LLH04_22180)	4717459..4718007	+	549	WP_057387874.1	DUF3158 family protein	-
LLH04_RS22170 (LLH04_22185)	4718055..4718885	+	831	WP_057387872.1	Rha family transcriptional regulator	-
LLH04_RS22175 (LLH04_22190)	4718915..4719403	+	489	WP_023104171.1	single-stranded DNA-binding protein	-
LLH04_RS22180 (LLH04_22195)	4719509..4719688	+	180	WP_057387869.1	hypothetical protein	-
LLH04_RS22185 (LLH04_22200)	4719932..4720099	+	168	WP_128729268.1	CrpP family ICE-associated protein	-
LLH04_RS22190 (LLH04_22205)	4720165..4720383	-	219	WP_023083218.1	hypothetical protein	-
LLH04_RS22195 (LLH04_22210)	4720400..4720594	-	195	WP_057387866.1	hypothetical protein	-
LLH04_RS22200 (LLH04_22215)	4720610..4721020	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LLH04_RS22205 (LLH04_22220)	4721020..4721250	-	231	WP_023132185.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LLH04_RS22210 (LLH04_22225)	4721569..4723488	+	1920	WP_057387864.1	type I DNA topoisomerase	-
LLH04_RS22215 (LLH04_22230)	4723497..4724249	+	753	WP_057387863.1	hypothetical protein	-
LLH04_RS22220 (LLH04_22235)	4724355..4726250	+	1896	WP_228214404.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4700084..4788419	88335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T222437 WP_003134109.1 NZ_CP086010:c4721020-4720610 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T222437 NZ_CP117852:3283463-3283747 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8468.35 Da
• Isoelectric Point: 4.2821

>AT222437 WP_023132185.1 NZ_CP086010:c4721250-4721020 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT222437 NZ_CP117852:3283225-3283473 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5