Detailed information of TA system TA222435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3661639..3662860
• Replicon: chromosome
• Accession: NZ_CP086010
• Organism: Pseudomonas aeruginosa isolate KB-PA_F19

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LLH04_RS17145
• Protein ID: WP_003102740.1
• Coordinates: 3662282..3662860 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LLH04_RS17140
• Protein ID: WP_003088112.1
• Coordinates: 3661639..3662193 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLH04_RS17115 (LLH04_17120)	3657207..3657758	+	552	WP_004343115.1	XRE family transcriptional regulator	-
LLH04_RS17120 (LLH04_17125)	3657755..3658153	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LLH04_RS17125 (LLH04_17130)	3658166..3658489	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
LLH04_RS17130 (LLH04_17135)	3658820..3659281	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LLH04_RS17135 (LLH04_17140)	3659286..3661481	+	2196	WP_228214376.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LLH04_RS17140 (LLH04_17145)	3661639..3662193	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LLH04_RS17145 (LLH04_17150)	3662282..3662860	+	579	WP_003102740.1	HD domain-containing protein	Toxin
LLH04_RS17150 (LLH04_17155)	3662969..3664156	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LLH04_RS17155 (LLH04_17160)	3664146..3666317	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
LLH04_RS17160 (LLH04_17165)	3666307..3667584	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T222435 WP_003102740.1 NZ_CP086010:3662282-3662860 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T222435 NZ_CP117852:2937341-2937444 [Salmonella enterica subsp. enterica serovar Mbandaka]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT222435 WP_003088112.1 NZ_CP086010:3661639-3662193 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT222435 NZ_CP117852:c2937446-2937303 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAAGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: A0A0C7CS07