Detailed information of TA system TA222432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1626351..1628046
• Replicon: chromosome
• Accession: NZ_CP086010
• Organism: Pseudomonas aeruginosa isolate KB-PA_F19

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: LLH04_RS07705
• Protein ID: WP_003112901.1
• Coordinates: 1626351..1627220 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: LLH04_RS07710
• Protein ID: WP_003112902.1
• Coordinates: 1627213..1628046 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLH04_RS07680 (LLH04_07680)	1622359..1623210	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
LLH04_RS07685 (LLH04_07685)	1623251..1624258	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
LLH04_RS07690 (LLH04_07690)	1624255..1625031	+	777	WP_003112898.1	ABC transporter permease	-
LLH04_RS07695 (LLH04_07695)	1625037..1625798	+	762	WP_003112899.1	SDR family oxidoreductase	-
LLH04_RS07700 (LLH04_07700)	1625812..1626342	+	531	WP_003112900.1	cupin domain-containing protein	-
LLH04_RS07705 (LLH04_07705)	1626351..1627220	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
LLH04_RS07710 (LLH04_07710)	1627213..1628046	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
LLH04_RS07715 (LLH04_07715)	1628033..1628830	+	798	WP_003112903.1	SDR family oxidoreductase	-
LLH04_RS07720 (LLH04_07720)	1628823..1630505	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
LLH04_RS07725 (LLH04_07725)	1630516..1631319	+	804	WP_003130116.1	aspartate dehydrogenase	-
LLH04_RS07730 (LLH04_07730)	1631331..1632815	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T222432 WP_003112901.1 NZ_CP086010:1626351-1627220 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T222432 NZ_CP117852:1298944-1299219 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGGACAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT222432 WP_003112902.1 NZ_CP086010:1627213-1628046 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT222432 NZ_CP117852:1298681-1298941 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCATTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1