Detailed information of TA system TA022242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3082125..3082804
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: TBFG_RS14615
• Protein ID: WP_003414059.1
• Coordinates: 3082125..3082541 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TBFG_RS14620
• Protein ID: WP_003414061.1
• Coordinates: 3082538..3082804 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS14595	3078177..3079079	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
TBFG_RS14600	3079148..3079900	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TBFG_RS14605	3080144..3080419	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TBFG_RS14610	3080416..3082038	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
TBFG_RS14615	3082125..3082541	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
TBFG_RS14620	3082538..3082804	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBFG_RS14625	3082830..3083225	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
TBFG_RS14630	3083222..3083491	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TBFG_RS14635	3083501..3084595	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
TBFG_RS14640	3084592..3085011	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TBFG_RS14645	3085085..3085564	-	480	WP_003414073.1	dihydrofolate reductase	-
TBFG_RS14650	3085635..3086435	-	801	WP_009939104.1	thymidylate synthase	-
TBFG_RS14655	3086591..3087328	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T22242 WP_003414059.1 NC_009565:c3082541-3082125 [Mycobacterium tuberculosis F11]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T22242 NC_009565:c3082541-3082125 [Mycobacterium tuberculosis F11]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT22242 WP_003414061.1 NC_009565:c3082804-3082538 [Mycobacterium tuberculosis F11]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT22242 NC_009565:c3082804-3082538 [Mycobacterium tuberculosis F11]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64