Detailed information of TA system TA222418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2453356..2453932
• Replicon: chromosome
• Accession: NZ_CP085987
• Organism: Salmonella enterica strain SZL 38

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LKX03_RS12715
• Protein ID: WP_001131963.1
• Coordinates: 2453645..2453932 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LKX03_RS12710
• Protein ID: WP_000063142.1
• Coordinates: 2453356..2453658 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKX03_RS12695 (2449866)	2449866..2452016	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
LKX03_RS12700 (2452111)	2452111..2452314	+	204	WP_000460616.1	YbdD/YjiX family protein	-
LKX03_RS12705 (2452325)	2452325..2453281	+	957	WP_000187839.1	GTPase	-
LKX03_RS12710 (2453356)	2453356..2453658	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LKX03_RS12715 (2453645)	2453645..2453932	-	288	WP_001131963.1	BrnT family toxin	Toxin
LKX03_RS12720 (2454201)	2454201..2455115	-	915	WP_000290512.1	restriction endonuclease	-
LKX03_RS12725 (2455313)	2455313..2458822	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T222418 WP_001131963.1 NZ_CP085987:c2453932-2453645 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T222418 NZ_CP117851:c2534682-2534437 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATCAACAGCAAAGCCTGCATGACCACCTGTTACTCACAAATCCCCAGCCAGCACCTTAAGGGTGACTGGCAGGAAGAAGC
GGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGA
GGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACGGG
GATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT222418 WP_000063142.1 NZ_CP085987:c2453658-2453356 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT222418 NZ_CP117851:2534447-2534858 [Salmonella enterica subsp. enterica serovar Mbandaka]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGTGGTCATGCAGGCTTTGCTGTTGATGTGG
CGGACGCTGACGCCGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAG
CTCAGGGCGAGCGAGTATGGGCGCGACATCCTGGCACTGCGCTCCCCTCTCCCCGGCCGGTGATGAGCAGCCGGCTGCCG
GGGCAGTATTTT

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01