Detailed information of TA system TA022238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2881924..2882555
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: TBFG_RS13540
• Protein ID: WP_003413174.1
• Coordinates: 2882178..2882555 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: TBFG_RS13535
• Protein ID: WP_003413167.1
• Coordinates: 2881924..2882181 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS13505	2877838..2878152	+	315	WP_009937839.1	hypothetical protein	-
TBFG_RS13510	2878448..2879659	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
TBFG_RS13515	2879786..2880445	+	660	WP_003900846.1	LppA family lipoprotein	-
TBFG_RS13520	2880442..2881104	+	663	WP_003900848.1	hypothetical protein	-
TBFG_RS13525	2881101..2881379	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
TBFG_RS13530	2881472..2881885	+	414	WP_003413164.1	PIN domain nuclease	-
TBFG_RS13535	2881924..2882181	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
TBFG_RS13540	2882178..2882555	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBFG_RS13545	2882571..2882945	-	375	WP_003413177.1	hypothetical protein	-
TBFG_RS13550	2883045..2883440	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
TBFG_RS13555	2883437..2883682	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
TBFG_RS13560	2884093..2884512	-	420	WP_003413190.1	A24 family peptidase	-
TBFG_RS13565	2884524..2885333	-	810	WP_003413193.1	shikimate dehydrogenase	-
TBFG_RS13570	2885330..2886583	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
TBFG_RS13575	2886576..2887088	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T22238 WP_003413174.1 NC_009565:2882178-2882555 [Mycobacterium tuberculosis F11]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T22238 NC_009565:2882178-2882555 [Mycobacterium tuberculosis F11]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT22238 WP_003413167.1 NC_009565:2881924-2882181 [Mycobacterium tuberculosis F11]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT22238 NC_009565:2881924-2882181 [Mycobacterium tuberculosis F11]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5