Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 43574..44164 Replicon plasmid pSZL30.1
Accession NZ_CP085982
Organism Salmonella enterica strain SZL 30

Toxin (Protein)


Gene name pemK Uniprot ID A0A241PXU0
Locus tag LJY16_RS23285 Protein ID WP_023994178.1
Coordinates 43574..43906 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A5T3Q5H6
Locus tag LJY16_RS23290 Protein ID WP_023994179.1
Coordinates 43907..44164 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJY16_RS23275 (LJY16_23275) 39848..42070 + 2223 WP_023994175.1 DEAD/DEAH box helicase -
LJY16_RS23280 (LJY16_23280) 42369..42974 - 606 Protein_53 IS481 family transposase -
LJY16_RS23285 (LJY16_23285) 43574..43906 - 333 WP_023994178.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LJY16_RS23290 (LJY16_23290) 43907..44164 - 258 WP_023994179.1 hypothetical protein Antitoxin
LJY16_RS23295 (LJY16_23295) 44775..45860 + 1086 WP_223155813.1 hypothetical protein -
LJY16_RS23300 (LJY16_23300) 45971..46477 + 507 WP_223155815.1 hypothetical protein -
LJY16_RS23305 (LJY16_23305) 46617..47636 + 1020 WP_223155817.1 hypothetical protein -
LJY16_RS23310 (LJY16_23310) 47640..48665 - 1026 WP_227945772.1 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / tet(A) faeI / faeH / faeF / faeE / faeD / faeC / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..272840 272840
- inside IScluster/Tn - - 42369..48677 6308


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11787.54 Da        Isoelectric Point: 9.3417

>T222379 WP_023994178.1 NZ_CP085982:c43906-43574 [Salmonella enterica]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGDFARTAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T222379 NZ_CP117835:247656-248006 [Alkalihalophilus pseudofirmus]
TTGATTGTAAAAAGAGGTGACGTTTATTTTGCCGACCTTTCACCTGTTGTTGGGTCTGAGCAGGGTGGGGTAAGACCTGT
TTTAATCATCCAAAATGATATTGGGAATCGTTTTAGTCCTACTGTCATTGTGGCTGCAATCACGGCACAAATTCAAAAGG
CAAAATTGCCGACTCATGTTGAAATCAATGCAAAAAGATACGGATTTGATCGTGATTCGGTCATTTTGCTAGAACAAGTA
AGAACAATTGATAAACAGCGCCTGACCGATAAAATCACCCATCTTGATGATGATATGATGACGAGGGTTAATGATGCTTT
GTTTATTAGTTTGGGATTAATTGACTTTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9374.86 Da        Isoelectric Point: 4.4354

>AT222379 WP_023994179.1 NZ_CP085982:c44164-43907 [Salmonella enterica]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWMDAPAV
GKEIL

Download         Length: 258 bp

>AT222379 NZ_CP117835:247368-247652 [Alkalihalophilus pseudofirmus]
ATGTTCGTGTCAGAACAGAGAACGAAACGCATTGTAGTGAATTTGCCACAACATTTGTTAAACGAGGTGGACGGTGTGAT
TCAACAAGAGAATGTAAATTACAGTGAGTTTTTATCGCAAGCAACGAAGCTATATCTTCGTGACCGAAAGAAGCGTCAAA
TTCGTGAAACGATGCAGCAAGGCTATATGGAAATGGCTAAAATCAACCTTAACCTTGCATCTGAAGCCTTTCTTGCCGAG
GAGGAAGCAGAGAACACCCTTGATCGCTTAGTAAGTGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241PXU0

Antitoxin

Source ID Structure
AlphaFold DB A0A5T3Q5H6

References