Detailed information of TA system TA022236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2867585..2868225
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: TBFG_RS13445
• Protein ID: WP_003412970.1
• Coordinates: 2867585..2868004 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: TBFG_RS13450
• Protein ID: WP_003412975.1
• Coordinates: 2868001..2868225 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS13420	2863170..2863892	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
TBFG_RS13425	2864409..2864636	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
TBFG_RS13430	2864633..2865034	+	402	WP_003412963.1	PIN domain-containing protein	-
TBFG_RS13435	2865069..2865989	-	921	WP_003412965.1	restriction endonuclease	-
TBFG_RS22505	2866330..2866575	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
TBFG_RS13440	2866634..2867584	+	951	WP_003911916.1	Lsr2 family protein	-
TBFG_RS13445	2867585..2868004	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBFG_RS13450	2868001..2868225	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
TBFG_RS13455	2868256..2871099	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
TBFG_RS13460	2871171..2871572	-	402	WP_003412981.1	hypothetical protein	-
TBFG_RS13465	2871572..2872042	-	471	WP_003899364.1	transcription antitermination factor NusB	-
TBFG_RS13470	2872045..2872608	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T22236 WP_003412970.1 NC_009565:c2868004-2867585 [Mycobacterium tuberculosis F11]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T22236 NC_009565:c2868004-2867585 [Mycobacterium tuberculosis F11]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT22236 WP_003412975.1 NC_009565:c2868225-2868001 [Mycobacterium tuberculosis F11]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT22236 NC_009565:c2868225-2868001 [Mycobacterium tuberculosis F11]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09