Detailed information of TA system TA222354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2956476..2957129
• Replicon: chromosome
• Accession: NZ_CP085978
• Organism: Mycobacterium avium subsp. avium strain FDAARGOS_1606

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LK466_RS13370
• Protein ID: WP_009975163.1
• Coordinates: 2956476..2956937 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: LK466_RS13375
• Protein ID: WP_003872881.1
• Coordinates: 2956941..2957129 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK466_RS13345 (LK466_13345)	2951844..2952182	-	339	WP_009975166.1	hypothetical protein	-
LK466_RS13350 (LK466_13350)	2952715..2952933	+	219	WP_016705560.1	hypothetical protein	-
LK466_RS13355 (LK466_13355)	2952968..2953354	-	387	WP_009975165.1	hypothetical protein	-
LK466_RS13360 (LK466_13360)	2953394..2954977	-	1584	WP_003872878.1	DUF4185 domain-containing protein	-
LK466_RS13365 (LK466_13365)	2955973..2956407	-	435	WP_023880080.1	hypothetical protein	-
LK466_RS13370 (LK466_13370)	2956476..2956937	-	462	WP_009975163.1	SRPBCC family protein	Toxin
LK466_RS13375 (LK466_13375)	2956941..2957129	-	189	WP_003872881.1	antitoxin	Antitoxin
LK466_RS13380 (LK466_13380)	2957191..2959563	-	2373	WP_227974711.1	cation-translocating P-type ATPase	-
LK466_RS13385 (LK466_13385)	2959560..2961146	-	1587	WP_009975161.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16627.13 Da
• Isoelectric Point: 9.5654

>T222354 WP_009975163.1 NZ_CP085978:c2956937-2956476 [Mycobacterium avium subsp. avium]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQNDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T222354 NZ_CP117821:c1104505-1103894 [Xylella fastidiosa]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGGGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGTGGATTGGC
TAGTATCATCCGCGAAGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT222354 WP_003872881.1 NZ_CP085978:c2957129-2956941 [Mycobacterium avium subsp. avium]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT222354 NZ_CP117821:c1104690-1104502 [Xylella fastidiosa]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATTGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18