Detailed information of TA system TA222353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2731469..2732033
• Replicon: chromosome
• Accession: NZ_CP085978
• Organism: Mycobacterium avium subsp. avium strain FDAARGOS_1606

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LK466_RS12310
• Protein ID: WP_227974694.1
• Coordinates: 2731469..2731840 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LK466_RS12315
• Protein ID: WP_011724021.1
• Coordinates: 2731827..2732033 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK466_RS12265 (LK466_12265)	2726634..2726957	-	324	WP_009975396.1	putative quinol monooxygenase	-
LK466_RS12270 (LK466_12270)	2727369..2727893	-	525	WP_227974692.1	C40 family peptidase	-
LK466_RS12275 (LK466_12275)	2728050..2728403	-	354	WP_003875396.1	hypothetical protein	-
LK466_RS12280 (LK466_12280)	2728473..2728856	-	384	WP_227974693.1	hypothetical protein	-
LK466_RS12285 (LK466_12285)	2728925..2729323	-	399	WP_009975395.1	VOC family protein	-
LK466_RS12290 (LK466_12290)	2729425..2729706	-	282	WP_029248407.1	hypothetical protein	-
LK466_RS12300 (LK466_12300)	2730126..2730863	+	738	WP_009975393.1	SDR family oxidoreductase	-
LK466_RS12305 (LK466_12305)	2730929..2731378	-	450	WP_024637459.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
LK466_RS12310 (LK466_12310)	2731469..2731840	-	372	WP_227974694.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK466_RS12315 (LK466_12315)	2731827..2732033	-	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LK466_RS12320 (LK466_12320)	2732202..2733146	-	945	WP_003875389.1	HNH endonuclease signature motif containing protein	-
LK466_RS12325 (LK466_12325)	2733346..2734419	-	1074	WP_009975391.1	redox-regulated ATPase YchF	-
LK466_RS12330 (LK466_12330)	2734550..2735743	+	1194	WP_080516334.1	hypothetical protein	-
LK466_RS12335 (LK466_12335)	2735760..2736758	-	999	WP_009975388.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13823.19 Da
• Isoelectric Point: 6.8444

>T222353 WP_227974694.1 NZ_CP085978:c2731840-2731469 [Mycobacterium avium subsp. avium]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVERHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T222353 NZ_CP117821:352912-353214 [Xylella fastidiosa]
ATGGAGAAAGGCACATCTCACTATAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTCGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT222353 WP_011724021.1 NZ_CP085978:c2732033-2731827 [Mycobacterium avium subsp. avium]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT222353 NZ_CP117821:353217-353618 [Xylella fastidiosa]
ATGAGATGTCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTACAAGAGCGAAACTAC
TAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCTTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCTTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGGTACGCCGGAAG
CTTGATCTCGACCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAACGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTCCT
AA