Detailed information of TA system TA222348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1344592..1345156
• Replicon: chromosome
• Accession: NZ_CP085977
• Organism: Mycobacterium avium subsp. avium strain FDAARGOS_1607

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: LK460_RS06590
• Protein ID: WP_003875391.1
• Coordinates: 1344785..1345156 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LK460_RS06585
• Protein ID: WP_011724021.1
• Coordinates: 1344592..1344798 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK460_RS06565 (LK460_06565)	1339867..1340865	+	999	WP_009975388.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LK460_RS06570 (LK460_06570)	1340882..1342075	-	1194	WP_080516334.1	hypothetical protein	-
LK460_RS06575 (LK460_06575)	1342206..1343279	+	1074	WP_009975391.1	redox-regulated ATPase YchF	-
LK460_RS06580 (LK460_06580)	1343479..1344423	+	945	WP_003875389.1	HNH endonuclease signature motif containing protein	-
LK460_RS06585 (LK460_06585)	1344592..1344798	+	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LK460_RS06590 (LK460_06590)	1344785..1345156	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK460_RS06595 (LK460_06595)	1345247..1345696	+	450	WP_024637459.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
LK460_RS06600 (LK460_06600)	1345762..1346499	-	738	WP_009975393.1	SDR family oxidoreductase	-
LK460_RS06610 (LK460_06610)	1346919..1347200	+	282	WP_029248407.1	hypothetical protein	-
LK460_RS06615 (LK460_06615)	1347302..1347700	+	399	WP_009975395.1	VOC family protein	-
LK460_RS06620 (LK460_06620)	1347769..1348152	+	384	WP_023884114.1	hypothetical protein	-
LK460_RS06625 (LK460_06625)	1348222..1348575	+	354	WP_003875396.1	hypothetical protein	-
LK460_RS06630 (LK460_06630)	1348732..1349256	+	525	WP_033713819.1	glycoside hydrolase	-
LK460_RS06635 (LK460_06635)	1349667..1349990	+	324	WP_009975396.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T222348 WP_003875391.1 NZ_CP085977:1344785-1345156 [Mycobacterium avium subsp. avium]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T222348 NZ_CP117820:1861678-1861971 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT222348 WP_011724021.1 NZ_CP085977:1344592-1344798 [Mycobacterium avium subsp. avium]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT222348 NZ_CP117820:1861379-1861675 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37