Detailed information of TA system TA222347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1119519..1120172
• Replicon: chromosome
• Accession: NZ_CP085977
• Organism: Mycobacterium avium subsp. avium strain FDAARGOS_1607

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LK460_RS05530
• Protein ID: WP_009975163.1
• Coordinates: 1119711..1120172 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: LK460_RS05525
• Protein ID: WP_003872881.1
• Coordinates: 1119519..1119707 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK460_RS05515 (LK460_05515)	1115502..1117088	+	1587	WP_009975161.1	serine hydrolase	-
LK460_RS05520 (LK460_05520)	1117085..1119457	+	2373	WP_009975162.1	cation-translocating P-type ATPase	-
LK460_RS05525 (LK460_05525)	1119519..1119707	+	189	WP_003872881.1	antitoxin	Antitoxin
LK460_RS05530 (LK460_05530)	1119711..1120172	+	462	WP_009975163.1	SRPBCC family protein	Toxin
LK460_RS05535 (LK460_05535)	1120241..1120675	+	435	WP_023880080.1	hypothetical protein	-
LK460_RS05540 (LK460_05540)	1121671..1123254	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
LK460_RS05545 (LK460_05545)	1123294..1123680	+	387	WP_009975165.1	hypothetical protein	-
LK460_RS05550 (LK460_05550)	1123715..1123933	-	219	WP_016705560.1	hypothetical protein	-
LK460_RS05555 (LK460_05555)	1124466..1124804	+	339	WP_009975166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16627.13 Da
• Isoelectric Point: 9.5654

>T222347 WP_009975163.1 NZ_CP085977:1119711-1120172 [Mycobacterium avium subsp. avium]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQNDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T222347 NZ_CP117820:1861379-1861675 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT222347 WP_003872881.1 NZ_CP085977:1119519-1119707 [Mycobacterium avium subsp. avium]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT222347 NZ_CP117820:1861678-1861971 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18