Detailed information of TA system TA222346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2195717..2196282
• Replicon: chromosome
• Accession: NZ_CP085976
• Organism: Blautia massiliensis strain FDAARGOS_1576

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U2EJ07
• Locus tag: LK422_RS10505
• Protein ID: WP_021651024.1
• Coordinates: 2195717..2196007 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LK422_RS10510
• Protein ID: WP_044961236.1
• Coordinates: 2196004..2196282 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK422_RS10475 (LK422_10475)	2190849..2191763	+	915	WP_014081148.1	HAMP domain-containing sensor histidine kinase	-
LK422_RS10480 (LK422_10480)	2192219..2192632	+	414	WP_014081146.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LK422_RS10485 (LK422_10485)	2192736..2193188	+	453	WP_021650281.1	hypothetical protein	-
LK422_RS10490 (LK422_10490)	2193199..2193660	+	462	WP_009296689.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LK422_RS10495 (LK422_10495)	2193689..2193979	+	291	WP_014081144.1	DUF3847 domain-containing protein	-
LK422_RS10500 (LK422_10500)	2194194..2195639	+	1446	WP_014081143.1	MobQ family relaxase	-
LK422_RS10505 (LK422_10505)	2195717..2196007	-	291	WP_021651024.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LK422_RS10510 (LK422_10510)	2196004..2196282	-	279	WP_044961236.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LK422_RS10515 (LK422_10515)	2196409..2196807	+	399	WP_014081140.1	cysteine-rich VLP domain-containing protein	-
LK422_RS10520 (LK422_10520)	2196921..2197655	+	735	WP_021651027.1	phage replisome organizer N-terminal domain-containing protein	-
LK422_RS10525 (LK422_10525)	2197652..2198515	+	864	WP_014081138.1	ATP-binding protein	-
LK422_RS10530 (LK422_10530)	2198559..2198753	+	195	WP_014081137.1	transposon-encoded TnpW family protein	-
LK422_RS10535 (LK422_10535)	2198831..2199196	+	366	WP_014081136.1	hypothetical protein	-
LK422_RS10540 (LK422_10540)	2199154..2200389	-	1236	WP_227945525.1	DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2186983..2199196	12213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11139.94 Da
• Isoelectric Point: 9.4307

>T222346 WP_021651024.1 NZ_CP085976:c2196007-2195717 [Blautia massiliensis]
MRKTKYTVKYTTAFKKDYKRAIKRGLKIELLEQVVALLSMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Nucleotide

• Length: 291 bp

>T222346 NZ_CP117820:1649850-1650137 [Xylella fastidiosa]
ATGGCTAAGTCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGCAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGCCGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCCGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10173.64 Da
• Isoelectric Point: 4.4692

>AT222346 WP_044961236.1 NZ_CP085976:c2196282-2196004 [Blautia massiliensis]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETIAAMLEAERIAKDPSVKGC
NDLDELFADLKK

Nucleotide

• Length: 279 bp

>AT222346 NZ_CP117820:1649615-1649857 [Xylella fastidiosa]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCCGGTCTGCGGCTACTTGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Toxin

• AlphaFold DB: U2EJ07