Detailed information of TA system TA222344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 22396..23360
• Replicon: chromosome
• Accession: NZ_CP085974
• Organism: Gordonia otitidis strain FDAARGOS_1600

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: LK459_RS00110
• Protein ID: WP_227921047.1
• Coordinates: 22396..22986 (-)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: H5TKZ9
• Locus tag: LK459_RS00115
• Protein ID: WP_007238396.1
• Coordinates: 22983..23360 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK459_RS00085 (LK459_00085)	17574..19130	-	1557	WP_227921041.1	cobyric acid synthase	-
LK459_RS00090 (LK459_00090)	19140..19535	-	396	WP_227921042.1	helix-turn-helix domain-containing protein	-
LK459_RS00095 (LK459_00095)	19665..20414	+	750	WP_227921044.1	SDR family oxidoreductase	-
LK459_RS00100 (LK459_00100)	20441..21469	-	1029	WP_227921046.1	hypothetical protein	-
LK459_RS00105 (LK459_00105)	21536..22399	-	864	WP_007238394.1	type I methionyl aminopeptidase	-
LK459_RS00110 (LK459_00110)	22396..22986	-	591	WP_227921047.1	zeta toxin family protein	Toxin
LK459_RS00115 (LK459_00115)	22983..23360	-	378	WP_007238396.1	hypothetical protein	Antitoxin
LK459_RS00120 (LK459_00120)	23552..24565	+	1014	WP_227921054.1	ABC transporter substrate-binding protein	-
LK459_RS00125 (LK459_00125)	24562..25641	+	1080	WP_007238398.1	iron ABC transporter permease	-
LK459_RS00130 (LK459_00130)	25635..26429	+	795	WP_227921056.1	heme ABC transporter ATP-binding protein	-
LK459_RS00135 (LK459_00135)	26611..27828	+	1218	WP_227921057.1	hypothetical protein	-
LK459_RS00140 (LK459_00140)	27883..28116	-	234	WP_227921062.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-155)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-60)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 21548.51 Da
• Isoelectric Point: 5.5789

>T222344 WP_227921047.1 NZ_CP085974:c22986-22396 [Gordonia otitidis]
VKRLDLVVGPNGSGKTTFVEQRLAPLLPGSVFVNADVIARDRWPDAPEAHSYEAARIASTIREALIEQGESFVAETVFSH
PSKLHLITRARRSGYVVVLHVVLVPVELSVARVAERVVEGGHFVPEEKIRGRYERLWALVSAAIGMVDHATVYDNSAPDV
TRIVARFVDGDHAGTAQWPSWAPDELIEITEPSLEI

Nucleotide

• Length: 591 bp

>T222344 NZ_CP117820:1378857-1379150 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13580.19 Da
• Isoelectric Point: 6.2422

>AT222344 WP_007238396.1 NZ_CP085974:c23360-22983 [Gordonia otitidis]
MTNVADKVTRFSSELVDAAASEGRREQRSARQQLEHWVRVGREVSDQRQVARRRVEAALAGRLPLGDLSIEEGAVFNAEI
AASLEESLATGNHVADRAARGLATVALDEQGRVVKHLPDGTQIVL

Nucleotide

• Length: 378 bp

>AT222344 NZ_CP117820:1378558-1378854 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Structures

Antitoxin

• AlphaFold DB: H5TKZ9