Detailed information of TA system TA222340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 295260..295791
• Replicon: chromosome
• Accession: NZ_CP085972
• Organism: Bordetella parapertussis strain FDAARGOS_1540

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: LK435_RS01480
• Protein ID: WP_003812626.1
• Coordinates: 295260..295526 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: LK435_RS01485
• Protein ID: WP_003816743.1
• Coordinates: 295513..295791 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK435_RS01455 (LK435_01455)	290387..290659	+	273	WP_003812615.1	hypothetical protein	-
LK435_RS01460 (LK435_01460)	290698..291408	-	711	WP_003812617.1	aquaporin Z	-
LK435_RS01465 (LK435_01465)	291519..292151	-	633	WP_003812619.1	PAS domain-containing protein	-
LK435_RS01470 (LK435_01470)	292446..293426	+	981	WP_010928297.1	tripartite tricarboxylate transporter substrate binding protein	-
LK435_RS01475 (LK435_01475)	293530..295029	+	1500	WP_225665838.1	M81 family metallopeptidase	-
LK435_RS01480 (LK435_01480)	295260..295526	+	267	WP_003812626.1	BrnT family toxin	Toxin
LK435_RS01485 (LK435_01485)	295513..295791	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
LK435_RS01495 (LK435_01495)	296085..296741	-	657	WP_010928295.1	phosphoribosylanthranilate isomerase	-
LK435_RS01500 (LK435_01500)	296756..298423	-	1668	WP_010928294.1	TRAP transporter large permease subunit	-
LK435_RS01505 (LK435_01505)	298426..299055	-	630	WP_010928293.1	TRAP transporter small permease subunit	-
LK435_RS01510 (LK435_01510)	299267..300361	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T222340 WP_003812626.1 NZ_CP085972:295260-295526 [Bordetella parapertussis]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T222340 NZ_CP117820:c742860-742564 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT222340 WP_003816743.1 NZ_CP085972:295513-295791 [Bordetella parapertussis]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT222340 NZ_CP117820:c742561-742268 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02