Detailed information of TA system TA222335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 1628758..1629289
• Replicon: chromosome
• Accession: NZ_CP085971
• Organism: Bordetella parapertussis strain FDAARGOS_1541

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: LK409_RS07775
• Protein ID: WP_003812626.1
• Coordinates: 1629023..1629289 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: LK409_RS07770
• Protein ID: WP_003816743.1
• Coordinates: 1628758..1629036 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK409_RS07745 (LK409_07745)	1624188..1625282	+	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-
LK409_RS07750 (LK409_07750)	1625494..1626123	+	630	WP_010928293.1	TRAP transporter small permease subunit	-
LK409_RS07755 (LK409_07755)	1626126..1627793	+	1668	WP_010928294.1	TRAP transporter large permease subunit	-
LK409_RS07760 (LK409_07760)	1627808..1628464	+	657	WP_010928295.1	phosphoribosylanthranilate isomerase	-
LK409_RS07770 (LK409_07770)	1628758..1629036	-	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
LK409_RS07775 (LK409_07775)	1629023..1629289	-	267	WP_003812626.1	BrnT family toxin	Toxin
LK409_RS07780 (LK409_07780)	1629520..1631019	-	1500	WP_225665838.1	M81 family metallopeptidase	-
LK409_RS07785 (LK409_07785)	1631123..1632103	-	981	WP_010928297.1	tripartite tricarboxylate transporter substrate binding protein	-
LK409_RS07790 (LK409_07790)	1632398..1633030	+	633	WP_003812619.1	PAS domain-containing protein	-
LK409_RS07795 (LK409_07795)	1633141..1633851	+	711	WP_003812617.1	aquaporin Z	-
LK409_RS07800 (LK409_07800)	1633890..1634162	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T222335 WP_003812626.1 NZ_CP085971:c1629289-1629023 [Bordetella parapertussis]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T222335 NZ_CP117818:c1123483-1122977 [Legionella pneumophila]
ATGGTTGATAATTCAATAATAACAATAAATGGCTGGGTTATTTTATGCCATCCTCTTTTTTTGCAACAGGTAGAGGCATT
GGCTAATGAGGTAGAAAAAGCAAAAGAAAAGGACCCTTCAAATTTTCATACACGAAACTCGACAAAAAAATTAGCAGCCA
TTGCTAAATTAGCTTTTGAAAAAATTCCAAACGATCCTGCAGCTACAGAATTTCGACAAGGATCAACATTAGGAAATAAC
CACAAACATTGGTTTCGAGCTAAATTTTTTCAGCAATATCGATTATTTTTTAGATACCATCAAGAAAGTAAAATAATTAT
TTATGCTTGGGTTAATGATGACAGTTCTAAAAGAGCATATGAAAGTAAAGCAGATGCTTATCATATTTTTCGAAAGATGC
TGGAAAATGGTCATCCTCCAGATAATTGGGATGAATTACTTAAAGAAGCAAATAACACTTCGTTAATGAAAAAATTGACT
ACCAGTTTGGATGCTTTGAAAAGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT222335 WP_003816743.1 NZ_CP085971:c1629036-1628758 [Bordetella parapertussis]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT222335 NZ_CP117818:c1123830-1123483 [Legionella pneumophila]
ATGGCTAGATTACTACAAACCGAATCCACCCTCACAGATCGTTATCAAACAACTGTACCAGATGCTGTGAGAAAAGCCCT
GCATCTAGGAAAAAGAGATAAAATTCGATACACCGTTCAATCTGACGGTCATGTTCTTATGACTCGAGCAGAGAAAGAGG
AAGATCCAGTAATTGCTGAATTTTTGAATTTCATTGCTAAAGACATTCAAAATAACCCCAAAAATCTGACAACTATAAAT
CAGGATTTAGTAACTCGCATCAACTCTTTAGTTGCAAATGTTGAAGTCAATTTAGATGAACCTTTAACCGAGGATGAACA
ATTAACTGAAAACCGAGTAAGTCGCTAA

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02